Multidimensional Analysis of Bronchoalveolar Lavage Cytokines and Mast Cell Proteases Reveals Interferon-γ as a Key Biomarker in Equine Asthma Syndrome
| Autor: | Kaori Uchiumi Davis, Elizabeth M. Lennon, Melissa T. Hines, Jane S. Woodrow, Zhiping Wang, Carla S. Sommardahl, Bente Flatland, Mary Katherine Sheats, Yancy Lo |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Proteases medicine.diagnostic_test 040301 veterinary sciences business.industry medicine.medical_treatment Chymase 04 agricultural and veterinary sciences Mast cell medicine.disease 3. Good health respiratory tract diseases 0403 veterinary science 03 medical and health sciences Cytokine medicine.anatomical_structure Bronchoalveolar lavage Immunopathology Immunology Medicine Tumor necrosis factor alpha business 030304 developmental biology Asthma |
| DOI: | 10.1101/2020.02.24.956573 |
| Popis: | Naturally-occurring equine asthma is an inflammatory lung disease characterized by chronic, partially reversible airway obstruction, pulmonary remodeling and lower airway inflammation. The cytokine profiles that distinguish asthma groups or subtypes in horses have not been systematically classified, and mast cell phenotypes, which, in human asthma, correlate with asthma type, lung function, and response to therapy, have not been well-described in horses. The purpose of this study was to: (1) compare mast cell protease mRNA expression between healthy and asthmatic horses, (2) analyze the cytokine profile present in BALF of currently defined equine asthma groups, and (3) use these data to evaluate potential biomarkers of defined asthma groups. Mast cell protease gene expression and select cytokine gene expression in cells isolated from BALF, and BALF multiplex cytokine assays were performed. Multidimensional analysis demonstrated that IFNγ differentiates severe from moderate asthma, and that TNFα and CXCL8 are key biomarkers of equine asthma subtype. Expression of chymase mRNA, a mast cell-specific protease, was significantly decreased in horses with mastocytic asthma. These results will help further define EAS immunopathology, which could improve understanding and definitions of asthma groups, while also potentially identify novel therapeutic strategies. |
| Databáze: | OpenAIRE |
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