MicroRNA-155 promotes bladder cancer growth by repressing the tumor suppressor DMTF1
| Autor: | Yang Peng, Wang He, Guangzheng Zhong, Peng Gu, Weibin Xie, Bei Liao, Bo Wang, Tianxin Lin, Jian Huang, Wen Dong, Xu Chen, Fu-ding Lu, Shaoxu Wu, Yun Xie, Li Huang |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Pathology medicine.medical_specialty Blotting Western Urinary Bladder microRNA-155 Mice Nude Apoptosis medicine.disease_cause Real-Time Polymerase Chain Reaction Immunoenzyme Techniques Mice microRNA medicine Tumor Cells Cultured Animals Humans Epigenetics RNA Messenger Cell Proliferation Neoplasm Staging Regulation of gene expression Mice Inbred BALB C Urinary bladder Bladder cancer Cell growth business.industry Reverse Transcriptase Polymerase Chain Reaction Cell Cycle Arf Cell cycle Middle Aged medicine.disease Prognosis Xenograft Model Antitumor Assays MicroRNAs medicine.anatomical_structure Oncology Urinary Bladder Neoplasms Cancer research bladder cancer Female Neoplasm Grading Neoplasm Recurrence Local business Carcinogenesis DMTF1 Transcription Factors Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Yang Peng 1, * , Wen Dong 1, 3, * , Tian-xin Lin 1, 3, * , Guang-zheng Zhong 1 , Bei Liao 2 , Bo Wang 1, 3 , Peng Gu 1 , Li Huang 1 , Yun Xie 1 , Fu-ding Lu 1 , Xu Chen 1 , Wei-bin Xie 1 , Wang He 1 , Shao-xu Wu 1 , Jian Huang 1 1 Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of China 2 Department of Medical Examination Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of China 3 Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, SunYat-Sen Memorial Hospital, SunYat-Sen University, Guangzhou, People’s Republic of China * These authors have contributed equally to this work Corresponding to: Wen Dong, e-mail: wendong1210@hotmail.com Jian Huang, e-mail: urolhj@sina.com Keywords: microRNA-155, bladder cancer, DMTF1, Arf, cell proliferation Received: November 24, 2014 Accepted: April 6, 2015 Published: April 18, 2015 ABSTRACT MicroRNA-155 (miR-155) is dysregulated in human cancers. In this study, we reported that miR-155 was over-expressed in bladder cancer tissues. We found that miR-155 promoted cell proliferation in vitro and tumorigenesis in vivo . MiR-155 directly reduced the expression of the tumor suppressor DMTF1. The expression of DMTF1 was decreased in bladder cancer tissues. Similar to the restoring miR-155 expression, knockdown of DMTF1 promoted cell growth and cell cycle progression, whereas DMTF1 over-expression rescued the effect of miR-155. Moreover, we investigated DMTF1-Arf-p53 pathway and found that DMTF1 worked in both p53-dependent and p53-independent manners. Taken together, our findings suggested that miR-155 functions as a tumor promoter in bladder cancer, which is partially through repressing DMTF1 expression. The identification of miR-155 and its novel target DMTF1 will be valuable in developing diagnostic markers and therapeutic applications for bladder cancer. |
| Databáze: | OpenAIRE |
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