Comparison of Electrical Stimulation Thresholds in Normal and Retinal Degenerated Mouse Retina
| Autor: | Mark S. Humayun, Duke V. Piyathaisere, Shih Jen Chen, Eyal Margalit, James D. Weiland, Eugene de Juan, Satoshi Suzuki |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Retinal Ganglion Cells Retinal degeneration Action Potentials Giant retinal ganglion cells Retinal ganglion Retina Mice chemistry.chemical_compound medicine Animals Pulse (signal processing) Chemistry Retinal Degeneration Intrinsically photosensitive retinal ganglion cells Retinal General Medicine Anatomy medicine.disease Electric Stimulation Mice Mutant Strains Retinal waves Mice Inbred C57BL Disease Models Animal Ophthalmology medicine.anatomical_structure Sensory Thresholds sense organs Microelectrodes Photic Stimulation Biomedical engineering |
| Zdroj: | Japanese Journal of Ophthalmology. 48:345-349 |
| ISSN: | 1613-2246 0021-5155 |
| Popis: | To compare the threshold for electrically elicited action potentials of retinal ganglion cells in normal mouse retina and photoreceptor degenerated (rd) mouse retina. Microelectrode recordings were made from retinal ganglion cells of normal and rd mice. Mice with a genetically based retinal degeneration (rd mice) were grown to the age of 16 weeks, when light-evoked responses could no longer be recorded. A bare wire was placed in the vitreous to stimulate the retina with charge-balanced current pulses. The following pulse shapes were investigated: single, square biphasic pulse, single sine wave, and biphasic pulse trains. Normal mice had significantly lower stimulus thresholds than rd mice for all pulse shapes. In normal and rd mice, short pulses were more efficient with respect to total charge used, but required a higher current. In normal mice, sine wave stimulation was significantly more efficient than a biphasic pulse of the same duration. No difference was noted between sine wave and square wave stimulation in rd mice. Pulse trains offered little benefit over single pulses. The amount of electrical charge required to elicit an action potential is dependent on the condition of the retina and the shape of the stimulus pulse used to deliver the charge. |
| Databáze: | OpenAIRE |
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