Cellular uptake and cytotoxicity of octahedral rhenium cluster complexes
| Autor: | Vladimir E. Fedorov, Soo Jin Choi, Konstantin A. Brylev, Jing Zhe Xu, Sung Jin Kim, Youn Soo Sohn, Jin-Ho Choy, Yuri V. Mironov |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Microscopy
Confocal biology Cell Survival Stereochemistry chemistry.chemical_element Biological Transport Rhenium biology.organism_classification Endocytosis Biochemistry Inorganic Chemistry HeLa chemistry Octahedron Cytoplasm Amphiphile Organometallic Compounds Cluster (physics) Humans Female Cytotoxicity Chelating Agents HeLa Cells |
| Zdroj: | Journal of Inorganic Biochemistry. 102:1991-1996 |
| ISSN: | 0162-0134 |
| DOI: | 10.1016/j.jinorgbio.2008.07.013 |
| Popis: | Cellular uptake behavior of a novel class of octahedral rhenium cluster compounds, hexahydroxo complexes K(4)[{Re(6)S(8)}(OH)(6)].8H(2)O (1) and K(4)[{Re(6)Se(8)}(OH)(6)].8H(2)O (2), was evaluated in human cervical adenocarcinoma HeLa cells. Confocal microscopy and flow cytometry studies demonstrated that rhenium cluster 1 was not internalized into cell, while rhenium cluster 2 was. Conjugation of a polymer to rhenium cluster 1, namely the derivative K(4)[{Re(6)S(8)}(OH)(5)L] (3) (L is amphiphilic diblock copolymer MPEG550-CH(2)CONH-GlyPheLeuGlyPheLeu-COO(-)), considerably enhanced cellular uptake in a concentration-dependent manner and was predominantly localized in the cytoplasm and nucleus upon incubation time. The uptake of rhenium cluster 2 was mediated by energy-dependent endocytosis, whereas rhenium cluster 3 was directly ingested into cells by cell-fusion-like mechanism. According to the cytotoxicity evaluation test, both rhenium clusters 2 and 3 did not exhibit acute cytotoxic effects up to 50 microM, at the practical concentration level of biological applications. It is, therefore, expected that the rhenium cluster complexes can be promising potential candidates as diagnostic agents for medical treatment. |
| Databáze: | OpenAIRE |
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