Cloning and polypeptide analysis of the leading region in F plasmid DNA transfer
| Autor: | Animesh Ray, Ron Skurray |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Genetics
Cloning DNA Bacterial Origin of transfer Cell Chromosome Mapping DNA Restriction Enzymes Biology Molecular biology Molecular Weight Restriction enzyme chemistry.chemical_compound F Factor medicine.anatomical_structure Plasmid Plasmid dna chemistry Conjugation Genetic medicine Escherichia coli Cloning Molecular Peptides Molecular Biology DNA |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0147-619X |
| Popis: | A segment of the F plasmid DNA, located between the origin of transfer and the primary F replication region, is the first to enter the recipient cell during conjugation.PstI,SalI, andSmaI restriction endonuclease sites have been mapped within this leading region in conjugational DNA transfer and chimeric plasmids carrying overlapping fragments of the region have been constructed. Analyses of polypeptides synthesized by maxicells carrying these chimeric plasmids have shown four new polypeptides ofMr 27,800, 23,100, 14,400, and 11,000 to be encoded by sequences within the leading region. |
| Databáze: | OpenAIRE |
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