Phytochemical study of Pilosocereus pachycladus and antibiotic-resistance modifying activity of syringaldehyde
| Autor: | Severino Gonçalves de Brito-Filho, Maria Denise Leite Ferreira, Isis Caroline da Silva Cirino, Milen Maria Magalhães de Souza Fernandes, Otemberg Souza Chaves, Jéssica Karina da Silva Maciel, Yanna Carolina Ferreira Teles, Maria de Fátima Vanderlei de Souza, Leonardo P. Felix, Raimundo Braz-Filho, Pedro Dantas Fernandes, José P. Siqueira-Júnior |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Cactaceae
Tetracycline medicine.drug_class Antibiotics lcsh:RS1-441 Biology medicine.disease_cause 01 natural sciences Syringaldehyde Forage cactus Microbiology lcsh:Pharmacy and materia medica chemistry.chemical_compound medicine Food science General Pharmacology Toxicology and Pharmaceutics Norfloxacin Phytochemical study 010405 organic chemistry Caatinga ecoregion 010401 analytical chemistry biology.organism_classification 0104 chemical sciences chemistry Resistant Staphylococcus aureus Staphylococcus aureus Efflux Antibacterial activity Bacteria medicine.drug |
| Zdroj: | Revista Brasileira de Farmacognosia, Vol 27, Iss 4, Pp 453-458 (2017) Revista Brasileira de Farmacognosia v.27 n.4 2017 Revista Brasileira de Farmacognosia Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA Revista Brasileira de Farmacognosia, Volume: 27, Issue: 4, Pages: 453-458, Published: AUG 2017 Revista Brasileira de Farmacognosia, Vol 27, Iss 4, Pp 453-458 |
| Popis: | Pilosocereus pachycladus F. Ritter, Cactaceae, popularly known as “facheiro”, is used as food and traditional medicine in Brazilian caatinga ecoregion. The plant is used to treat prostate inflammation and urinary infection. The present work reports the first secondary metabolites isolated from P. pachycladus. Therefore, the isolated compound 4-hydroxy-3,5-dimethoxy benzaldehyde (syringaldehyde) was evaluated as modulator of Staphylococcus aureus pump efflux-mediated antibiotic resistance. The isolation of compounds was performed using chromatographic techniques and the structural elucidation was carried out by spectroscopic methods. In order to evaluate syringaldehyde ability to modulate S. aureus antibiotic resistance, its minimum inhibitory concentrations (μg/ml) was first determinate, then, the tested antibiotics minimum inhibitory concentrations were determined in the presence of the syringaldehyde in a sub-inhibitory concentration. The chromatographic procedures led to isolation of twelve compounds from P. pachycladus including fatty acids, steroids, chlorophyll derivatives, phenolics and a lignan. The syringaldehyde did not show any antibacterial activity at 256 μg/ml against S. aureus. On the other hand the compound was able to reduce the antibiotic concentration (tetracycline, norfloxacin, ethidium bromide) required to inhibit the growth of drug-resistant bacteria, showing the ability of syringaldehyde of inhibiting the efflux pump on these bacteria. Keywords: Forage cactus, Caatinga ecoregion, Cactaceae, Phytochemical study, Antibacterial activity, Resistant Staphylococcus aureus |
| Databáze: | OpenAIRE |
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