Application of DNA Fingerprinting to Investigation of Genetic Relationships between Laboratory Rabbit Strains
| Autor: | Soichi Tsuji, Hidehiko Umezawa, Toshiki Sudo, Nobuo Goto, Hajime Yazawa, Hideyuki Mannen, Makoto Mizutani, Masashi Shiomi |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Genetics General Veterinary biology Strain (biology) Dendrogram General Medicine Japanese white rabbit biology.organism_classification DNA Fingerprinting Molecular biology General Biochemistry Genetics and Molecular Biology Rats Bacteriophage Mice Inbred strain DNA profiling Laboratory rabbit Animals Laboratory Animals Female Animal Science and Zoology Rabbits Genetic monitoring |
| Zdroj: | Experimental Animals. 42:337-342 |
| ISSN: | 1881-7122 0007-5124 |
| DOI: | 10.1538/expanim1978.42.3_337 |
| Popis: | It is well known that laboratory rabbits are not controlled genetically like laboratory mice and rats. In order to test the usefulness of DNA fingerprinting in investigation of genetic uniformity of the laboratory rabbits strains and their relationships, we applied DNA fingerprinting using bacteriophage M13 probe to five strains (2 inbreds (JWY-NIBS and DuY-NIBS) and 3 outbreds (JW-NIBS, Icl:JW and WHHL)). DNA fingerprints of 2 inbred strains showed the same banding patterns within each strain but the strain-specific patterns. Although there were no rabbits showing the same banding patterns in 3 outbred strains, average percent differences (APD) were 13.7 to 18.6. A dendrogram based on APD of DNA fingerprints was constructed by 2 large clusters, JW group and DuY. The dendrogram was essentially similar to that based on rabbit mandible measurements. These results suggest that DNA fingerprinting is available not only for the genetic monitoring of the laboratory rabbit strains but also for the investigation of their genetic relationships. |
| Databáze: | OpenAIRE |
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