Loss of Plastoglobule Kinases ABC1K1 and ABC1K3 Causes Conditional Degreening, Modified Prenyl-Lipids, and Recruitment of the Jasmonic Acid Pathway
| Autor: | Mason J. Appel, Lalit Ponnala, Elden Rowland, Anton Poliakov, Ryan P. McQuinn, Giulia Friso, Qi Sun, Peter K. Lundquist, Stuart B. Krasnoff, L. Giacomelli, Klaas J. van Wijk |
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| Rok vydání: | 2013 |
| Předmět: |
Proteomics
Chloroplasts Light Plastoglobule Immunoblotting Mutant Arabidopsis Cyclopentanes Plant Science Protein Serine-Threonine Kinases Biology Thylakoids Gene Expression Regulation Enzymologic Mass Spectrometry chemistry.chemical_compound Gene Expression Regulation Plant Oxylipins Jasmonate Plastid Research Articles Arabidopsis Proteins Pigmentation Reverse Transcriptase Polymerase Chain Reaction Jasmonic acid Wild type food and beverages Cell Biology Biosynthetic Pathways Plant Leaves Chloroplast Microscopy Electron Oxidative Stress chemistry Biochemistry Multiprotein Complexes Thylakoid Mutation lipids (amino acids peptides and proteins) Electrophoresis Polyacrylamide Gel Protein Kinases Protein Binding |
| Zdroj: | The Plant Cell. 25:1818-1839 |
| ISSN: | 1532-298X 1040-4651 |
| Popis: | Plastoglobules (PGs) are plastid lipid-protein particles. This study examines the function of PG-localized kinases ABC1K1 and ABC1K3 in Arabidopsis thaliana. Several lines of evidence suggested that ABC1K1 and ABC1K3 form a protein complex. Null mutants for both genes (abc1k1 and abc1k3) and the double mutant (k1 k3) displayed rapid chlorosis upon high light stress. Also, k1 k3 showed a slower, but irreversible, senescence-like phenotype during moderate light stress that was phenocopied by drought and nitrogen limitation, but not cold stress. This senescence-like phenotype involved degradation of the photosystem II core and upregulation of chlorophyll degradation. The senescence-like phenotype was independent of the EXECUTER pathway that mediates genetically controlled cell death from the chloroplast and correlated with increased levels of the singlet oxygen–derived carotenoid β-cyclocitral, a retrograde plastid signal. Total PG volume increased during light stress in wild type and k1 k3 plants, but with different size distributions. Isolated PGs from k1 k3 showed a modified prenyl-lipid composition, suggesting reduced activity of PG-localized tocopherol cyclase (VTE1), and was consistent with loss of carotenoid cleavage dioxygenase 4. Plastid jasmonate biosynthesis enzymes were recruited to the k1 k3 PGs but not wild-type PGs, while pheophytinase, which is involved in chlorophyll degradation, was induced in k1 k3 and not wild-type plants and was localized to PGs. Thus, the ABC1K1/3 complex contributes to PG function in prenyl-lipid metabolism, stress response, and thylakoid remodeling. |
| Databáze: | OpenAIRE |
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