Spectroscopic Studies of the Interaction of Ferrous Bleomycin with DNA
| Autor: | Erik C. Wasinger, Keith O. Hodgson, Britt Hedman, Marina S. Chow, Ekaterina Y. Shishova, Kelly Loeb Zaleski, Andrea Decker, Jyllian N. Kemsley, Edward I. Solomon |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Azides
Circular dichroism Stereochemistry Guanine Oligonucleotides Cleavage (embryo) Biochemistry Catalysis Bleomycin chemistry.chemical_compound Colloid and Surface Chemistry Animals Binding site Binding Sites biology Oligonucleotide Chemistry Circular Dichroism Electron Spin Resonance Spectroscopy Spectrometry X-Ray Emission Active site DNA General Chemistry Oxygen Deoxyribose biology.protein Cattle |
| Zdroj: | Journal of the American Chemical Society. 125:10810-10821 |
| ISSN: | 1520-5126 0002-7863 |
| Popis: | Bleomycin is an antibiotic used in cancer chemotherapy for its ability to achieve both single- and double-strand cleavage of DNA through abstraction of the deoxyribose C4'-H. Magnetic circular dichroism (MCD) and X-ray absorption (XAS) spectroscopies have been used to study the interaction of the biologically relevant FeIIBLM complex with DNA. Calf thymus DNA was used as the substrate as well as short oligonucleotides, including one with a preferred 5'-G-pyrimidine-3' cleavage site [d(GGAAGCTTCC)2] and one without [d(GGAAATTTCC)2]. DNA binding to FeIIBLM significantly perturbs the FeII active site, resulting in a change in intensity ratio of the d d transitions and a decrease in excited-state orbital splitting (5Eg). Although this effect is somewhat dependent on length and composition of the oligonucleotide, it is not correlated to the presence of a 5'-G-pyrimidine-3' cleavage site. No effect is observed on the charge-transfer transitions, indicating that the H-bonding recognition between the pyrimidine and guanine base does not perturb Fe-pyrimidine backbonding. Azide binding studies indicate that FeIIBLM bound to either oligomer has the same affinity for N3-. Parallel studies of BLM structural derivatives indicate that FeIIiso-PEPLM, in which the carbamoyl group is shifted on the mannose sugar, forms the same DNA-bound species as FeIIBLM. In contrast, FeIIDP-PEPLM, in which the -aminoalanine group is absent, forms a new species upon DNA binding. These data are consistent with a model in which the primary amine from the -aminoalanine is an FeII ligand and the mannose carbamoyl provides either a ligand to the FeII or significant second-sphere effects on the FeII site; intercalation of the bithiazole tail into the double helix likely brings the metal-bound complex close enough to the DNA to create steric interactions that remove the sugar groups from interaction with the FeII. The fact that the FeII active site is perturbed regardless of DNA sequence is consistent with the fact that cleavage is observed for both 5'-GC-3' and nonspecific oligomers and indicates that different reaction coordinates may be active, depending on orientation of the deoxyribose C4'-H. |
| Databáze: | OpenAIRE |
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