Intracellular persistence of Escherichia coli in urinary bladders from mecillinam-treated mice
| Autor: | Carsten Struve, Karen A. Krogfelt, M. B. Kerrn, Jens Blom, Niels Frimodt-Møller |
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| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
medicine.drug_class Urinary system Urinary Bladder Antibiotics Anti-Infective Agents Urinary Colony Count Microbial urologic and male genital diseases medicine.disease_cause Microbiology Mice Escherichia coli medicine Animals Humans Pharmacology (medical) Mecillinam Antibacterial agent Pharmacology Urinary bladder biology Amdinocillin biology.organism_classification Enterobacteriaceae Microscopy Electron Infectious Diseases medicine.anatomical_structure Bacteria medicine.drug |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 55:383-386 |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dki002 |
| Popis: | Objectives: It has been suggested recently that intracellular bacteria surviving antibiotic treatment might serve as a reservoir for recurrent infection. The purpose of this study was to directly examine the location of Escherichia coli bacteria in the mouse bladder after treatment with mecillinam. Methods: The bladders were studied by use of colony counts, in situ hybridization and electron microscopy. Results: The bacterial counts in the bladder remained � 10 3–4 cfu/bladder even after mecillinam treatment had finished, and re-growth in the urine was observed. In the bladder epithelium from treated mice, bacteria cells were occasionally seen, presumably representing intracellularly located bacteria. Conclusions: This is the first in vivo study indicating that during mecillinam treatment E. coli cells can penetrate the mouse bladder epithelium and persist. |
| Databáze: | OpenAIRE |
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