Spatial and Temporal Control of Gene Expression in Drosophila Using the Inducible GeneSwitch GAL4 System. I. Screen for Larval Nervous System Drivers
| Autor: | Haig Keshishian, Thomas Osterwalder, Gunisha K. Singh, Louise F.B. Nicholson, Gregg Roman, Ronald L. Davis |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Nervous system
Saccharomyces cerevisiae Proteins Transgene Neuromuscular Junction Sensory system Investigations Nervous System Upstream activating sequence Gene expression Genetics medicine Animals Neurons Afferent Transgenes Drosophila Motor Neurons biology Effector Muscles Larval nervous system Brain biology.organism_classification Molecular biology DNA-Binding Proteins Mifepristone Mutagenesis Insertional medicine.anatomical_structure Drosophila melanogaster Enhancer Elements Genetic Phenotype Gene Expression Regulation Larva Neuroglia Genes Switch Transcription Factors |
| Popis: | There is a critical need for genetic methods for the inducible expression of transgenes in specific cells during development. A promising approach for this is the GeneSwitch GAL4 system of Drosophila. With GeneSwitch GAL4 the expression of upstream activating sequence (UAS) effector lines is controlled by a chimeric GAL4 protein that becomes active in the presence of the steroid RU486 (mifepristone). To improve the utility of this expression system, we performed a large-scale enhancer-trap screen for insertions that yielded nervous system expression. A total of 204 GeneSwitch GAL4 lines with various larval expression patterns in neurons, glia, and/or muscle fibers were identified for chromosomes I–III. All of the retained lines show increased activity when induced with RU486. Many of the lines reveal novel patterns of sensory neurons, interneurons, and glia. There were some tissue-specific differences in background expression, with muscles and glia being more likely to show activity in the absence of the inducing agent. However, >90% of the neuron-specific driver lines showed little or no background activity, making them particularly useful for inducible expression studies. |
| Databáze: | OpenAIRE |
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