Improved specificity of Trypanosoma cruzi identification by polymerase chain reaction using an oligonucleotide derived from the amino-terminal sequence of a Tc24 protein
| Autor: | A. Guevara-Espinoza, A. Taibi, Ali Ouaissi, Bilal Yahiaoui, R. Schöneck |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Trypanosoma rangeli
Antigenicity Recombinant Fusion Proteins Trypanosoma cruzi Blotting Western Molecular Sequence Data Protozoan Proteins Antibodies Protozoan Polymerase Chain Reaction Immunoglobulin G law.invention Mice Antigen law Animals Polymerase chain reaction Mice Inbred BALB C biology Base Sequence Oligonucleotide biology.organism_classification Molecular biology Peptide Fragments Blotting Southern Infectious Diseases Recombinant DNA biology.protein Animal Science and Zoology Parasitology Oligonucleotide Probes |
| Zdroj: | Parasitology. 111 |
| ISSN: | 0031-1820 |
| Popis: | SUMMARYIn the present study, the diagnostic value ofTrypanosoma cruzirecombinant protein (Tc24) was examined. Although antibodies against Tc24 were detected during natural and experimentalT. cruziinfections, specificity studies revealed that sera fromT. rangeli-infected mice also recognized to some extent Tc24 protein. In addition, sera from Tc24-immunized mice reacted against a 21 kDa polypeptide inT. rangeliextracts. Detailed analysis of the antibody response against 20—40 peptide localized in the Tc24 amino-terminal domain suggests that this sequence is not expressed byT. rangeli21 kDa antigen. Therefore, the PCR reaction using oligonucleotides corresponding to a 20–26 peptide clearly demonstrated the specificity of the oligoprobes forT. cruziidentification. Positive signals were also found when using blood samples fromT. cruzi-infected mice. Taken together, these results suggest that the PCR-based 20–26 assay may be useful in the specific diagnosis of Chagas' disease. |
| Databáze: | OpenAIRE |
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