Increased Expression of Macrophage Migration Inhibitory Factor in the Nucleus of the Solitary Tract Attenuates Renovascular Hypertension in Rats

Autor: Hongwei Li, José Vanderlei Menani, Guilherme Fleury Fina Speretta, Daniel Penteado Martins Dias, Rafaela Moreira Barbosa, Debora S. A. Colombari, Colin Sumners, Prashant J. Ruchaya, Eduardo Colombari
Přispěvatelé: Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), Southern Med Univ, Univ Florida
Rok vydání: 2017
Předmět:
Male
medicine.medical_specialty
Mean arterial pressure
hypertension
Drinking Behavior
angiotensin II
030204 cardiovascular system & hematology
Baroreflex
brainstem
Renovascular hypertension
Rats
Sprague-Dawley
Eating
03 medical and health sciences
Renal Artery
0302 clinical medicine
Heart Rate
Internal medicine
Solitary Nucleus
Internal Medicine
medicine
Animals
baroreflex
Arterial Pressure
Gene Knock-In Techniques
Macrophage Migration-Inhibitory Factors
Angiotensin II receptor type 1
AT1 receptor
business.industry
Solitary tract
blood pressure
respiratory system
medicine.disease
Angiotensin II
Rats
Intramolecular Oxidoreductases
Disease Models
Animal
Hypertension
Renovascular
Endocrinology
Blood pressure
nervous system
Original Article
Macrophage migration inhibitory factor
business
2K1C
030217 neurology & neurosurgery
circulatory and respiratory physiology
Zdroj: Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1941-7225
0895-7061
DOI: 10.1093/ajh/hpx001
Popis: Made available in DSpace on 2018-11-26T17:33:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-04-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) NIH BACKGROUND Macrophage migration inhibitory factor (MIF) is an intracellular inhibitory regulator of the actions of angiotensin II in the central nervous system. Renovascular hypertensive 2-kidney, 1-clip (2K1C) rats have an increased activity of the renin-angiotensin system and a decrease in baroreflex function compared to normotensive (NT) rats. In the present study, we tested the effects of MIF overexpression within the nucleus of the solitary tract (NTS), a key brainstem region for cardiovascular regulation, on the development of hypertension, on baroreflex function, and on water and food intake in 2K1C rats. METHODS Holtzman NT rats received a silver clip around the left renal artery to induce 2K1C hypertension. Three weeks later, rats were microinjected in the NTS with AAV2-CBA-MIF, to increase the expression of MIF, or with the control vector AAV2-CBA-enhanced green fluorescent protein. Mean arterial pressure (MAP) and heart rate were recorded by telemetry. Baroreflex function was tested, and water and food intake were also measured. RESULTS Increasing MIF expression in the NTS of 2K1C rats attenuated the development of hypertension, reversed the impairment of baroreflex function, and reduced the increase in water intake. In contrast to 2K1C rats, similar increases in MIF expression in the NTS of NT rats produced no changes in baseline MAP, baroreflex function, or water intake. CONCLUSIONS These results indicate that an increased expression of MIF within the NTS attenuates the development of hypertension and restores the baroreflex function in 2K1C rats. Sao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, Brazil Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Physiol, Ribeirao Preto, SP, Brazil Southern Med Univ, Sch Biotechnol, Guangzhou, Guangdong, Peoples R China Univ Florida, Coll Med, Dept Physiol & Funct Genom, Gainesville, FL USA Sao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, Brazil CNPq: 473108/2011-9 CNPq: 304918/2011-3 FAPESP: 2011/50770-1 FAPESP: 2015/23467-7 NIH: HL-076803
Databáze: OpenAIRE
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