Demonstration of the cell cycle positions of taxol-induced 'asters' and 'bundles' by sequential measurements of tubulin immunofluorescence, DNA content, and autoradiographic labeling of taxol-sensitive and -resistant cells
| Autor: | John Roberts, Ross C. Donehower, David C. Allison, Eric K. Rowinsky, Joel Robertson |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Histology
Paclitaxel Drug Resistance Fluorescent Antibody Technique Mitosis macromolecular substances Immunofluorescence Microtubules Cell Line chemistry.chemical_compound Alkaloids Leukemia Promyelocytic Acute Tubulin medicine Humans Feulgen stain biology medicine.diagnostic_test Cell Cycle DNA Cell cycle Antineoplastic Agents Phytogenic Staining Cell biology Leukemia Myeloid Acute Microscopy Electron chemistry biology.protein Autoradiography Anatomy Thymidine |
| Zdroj: | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 37(11) |
| ISSN: | 0022-1554 |
| Popis: | We used reliable and relatively inexpensive equipment to make sequential sets of measurements of antitubulin immunofluorescence, Feulgen staining, and autoradiography on the same cells. This was done to evaluate tubulin conformations, DNA content, and [3H]-thymidine incorporation in cell lines sensitive (HL60) and resistant (K562) to the novel anti-tubulin chemotherapeutic agent taxol. Numbers of cells with microtubule bundles have been found to correlate with sensitivity to taxol by clonogenic assay for several leukemic cell lines. We have found that cells with "asters" produced by taxol exposure are in mitosis and that cells with taxol-induced "bundles" are in G0/G1, S, and G2 phases. We further found that S-phase cells with microtubule bundles in both sensitive (HL60) and resistant (K562) cell lines were able to incorporate [3H]-thymidine after 4-hr exposure to taxol. As microtubule bundles and asters occur in cells of the same cell cycle phases in both lines, we conclude that the greater frequency of cells with microtubule bundles reported for sensitive cells after taxol treatment cannot result from drug exclusion nor from different effects of the drug on cell microtubules in these two leukemic lines. |
| Databáze: | OpenAIRE |
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