Maternal N-Carbamylglutamate Supplementation during Early Pregnancy Enhances Embryonic Survival and Development through Modulation of the Endometrial Proteome in Gilts
| Autor: | Hexiao Shen, Shiyan Qiao, Qian Peng, Jinlong Zhu, Haiyi Zhao, Shenming Zeng, Xiangfang Zeng |
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| Rok vydání: | 2015 |
| Předmět: |
Proteomics
China medicine.medical_specialty Litter Size Early Pregnancy Loss Sus scrofa Protein metabolism Embryonic Development Medicine (miscellaneous) Biology Nitric Oxide Endometrium Random Allocation chemistry.chemical_compound Glutamates Pregnancy Internal medicine medicine Animals Amino Acids Crosses Genetic Fetus Nutrition and Dietetics Embryogenesis Gene Expression Regulation Developmental Embryo Fetal Resorption Maternal Nutritional Physiological Phenomena medicine.disease Placentation Endocrinology medicine.anatomical_structure chemistry Dietary Supplements Gestation Female Biomarkers |
| Zdroj: | The Journal of Nutrition. 145:2212-2220 |
| ISSN: | 0022-3166 |
| Popis: | BACKGROUND Early pregnancy loss is a major concern in humans and animals. N-carbamylglutamate (NCG) has been found to enhance embryonic survival during early pregnancy in rats. However, little is known about the key factors in the endometrium involved in the improvement of embryonic implantation and development induced by maternal NCG supplementation. OBJECTIVES Our objectives were to investigate whether NCG supplementation during early gestation enhanced embryonic survival and development in gilts and to uncover the related factors using the approach of endometrium proteome analysis with isobaric tags for relative and absolute quantification (iTRAQ). METHODS Uteruses and embryos/fetuses were obtained on days 14 and 28 of gestation from gilts fed a basal diet that was or was not supplemented with 0.05% NCG. The iTRAQ-based quantitative proteomics approach was performed to explore the endometrium proteome altered by NCG supplementation. RESULTS Maternal NCG supplementation significantly increased the number of total fetuses and live fetuses on day 28 of gestation by 1.32 and 1.29, respectively (P < 0.05), with a significant decrease in embryonic mortality (P < 0.05). iTRAQ results indicated that a total of 59 proteins showed at least 2-fold differences (P < 0.05), including 52 proteins that were present at higher abundance and 7 proteins present at lower abundance in NCG-supplemented gilts. The differentially expressed proteins primarily are involved in cell adhesion, energy metabolism, lipid metabolism, protein metabolism, antioxidative stress, and immune response. On day 14 of gestation, several proteins closely related to embryonic implantation and development, such as integrin-αv, integrin-β3, talin, and endothelial nitric oxide synthase, were upregulated (3.7-, 4.1-, 2.4-, and 5.4-fold increases, respectively) by NCG supplementation. CONCLUSION To our knowledge, our results provide the first evidence that altered abundance of the endometrial proteome induced by NCG supplementation is highly associated with the improvement of embryonic survival and development in gilts. |
| Databáze: | OpenAIRE |
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