Reference Range of Platelet Delta Granules in the Pediatric Age Group: An Ultrastructural Study of Platelet Whole Mount Preparations from Healthy Volunteers
| Autor: | Bryce A. Kerlin, Minhaj Uddin, Peter B. Baker, Razan F. Alkhoury, Victoria Sorokin, David J. Thornton, Sura M. Al-Rawabdeh, Samir B. Kahwash, Ronald H. Houston, Han Yin, Carl P. Boesel, Sarah H. O'Brien |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Blood Platelets
Male Pathology medicine.medical_specialty Percentile Adolescent Platelet disorder Reference range 030204 cardiovascular system & hematology Cytoplasmic Granules Gastroenterology Pathology and Forensic Medicine 03 medical and health sciences Young Adult 0302 clinical medicine Microscopy Electron Transmission Reference Values Internal medicine Healthy volunteers medicine Humans Platelet Child Whole mount business.industry Granule (cell biology) Infant Pediatric age General Medicine Healthy Volunteers 030220 oncology & carcinogenesis Child Preschool Pediatrics Perinatology and Child Health Female business |
| Zdroj: | Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society. 19(6) |
| ISSN: | 1093-5266 |
| Popis: | This study sought to determine delta granule normal ranges for children and to validate methodology for the appropriate diagnosis of delta granule deficiency (storage pool disease) by using the whole-mount technique in electron microscopy. Specimens obtained from 40 healthy volunteers (2 months of age through 21 years old, 21 females and 19 males) were tested. Results showed dense granules/platelet (DG/Plt) ranged from 1.78 to 5.25. The 5th percentile was 1.96 DG/Plt with an overall mean ± SEM 3.07 ± 0.12 DG/Plt. In comparison, a previously published lower cutoff value, 3.68 DG/Plt, was significantly higher than the mean from our volunteers ( P < 0.0001). We found no variability in dense granules/platelet based on race or sex and no significant variation by age subgroup. Pending wider studies, the value of 2 DG/Plt is a more appropriate lower limit of normal. In the absence of wider studies (in healthy volunteers and patients), laboratories should consider establishing their own reference ranges. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|