Detection and identification of Lactobacillus delbrueckii subsp. lactis bacteriophages by PCR
| Autor: | Lucia Comaschi, Giorgio Giraffa, Miriam Zago, Domenico Carminati, Angela De Lorentiis |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Restriction Mapping Bacillus Phages Polymerase Chain Reaction Virus law.invention Microbiology Bacteriophage law Cheese Lactobacillus Animals Cluster Analysis Bacteriophages Polymerase chain reaction Lactobacillus delbrueckii biology Base Sequence Thermophile Gene Amplification food and beverages General Medicine Lactobacillaceae biology.organism_classification Milk Proteins Milk Whey Proteins Lytic cycle DNA Viral Food Microbiology Animal Science and Zoology Cattle Sequence Alignment Bacteria Food Science |
| Zdroj: | The Journal of dairy research. 73(2) |
| ISSN: | 0022-0299 |
| Popis: | A sensitive PCR method amplifying an internal fragment of the major tail protein gene was developed to detect Lactobacillus delbrueckii subsp. lactis lytic bacteriophages in undefined, thermophilic whey starters used in Italy for production of Grana and Provolone cheeses. PCR was applied to several lytic Lb. delbrueckii subsp. lactis bacteriophages, which were highly diverse according to restriction analysis and phage host range. PCR detected the presence of phages in two out of 11 cultures, when applied to whey starters for Grana Padano cheese sampled from different cheese plants. The presence of actively growing phages in infected cultures was confirmed by traditional test. The PCR method proved to be useful to screen for the presence of Lb. delbrueckii subsp. lactis phages in thermophilic whey starters. |
| Databáze: | OpenAIRE |
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