Molecular profiling of fungal communities in moisture damaged buildings before and after remediation - a comparison of culture-dependent and culture-independent methods

Autor: Helena Rintala, Aino Nevalainen, Petri Auvinen, Lars Paulin, Ulla Lignell, Teija Meklin, Miia Pitkäranta, Anne Hyvärinen
Přispěvatelé: Institute of Biotechnology, DNA Sequencing and Genomics
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Microbiology (medical)
Library
Environmental remediation
Culture dependent
Microorganism
education
lcsh:QR1-502
DIVERSITY
Colony Count
Microbial
010501 environmental sciences
Biology
01 natural sciences
Microbiology
Polymerase Chain Reaction
lcsh:Microbiology
RELATIVE MOLDINESS INDEX
03 medical and health sciences
MOLD
Botany
HOME REMEDIATION
Humans
EXPOSURE
Internal transcribed spacer
1183 Plant biology
microbiology
virology
Phylogeny
0105 earth and related environmental sciences
0303 health sciences
Moisture
MICROBIAL COMMUNITY
030306 microbiology
business.industry
Construction Materials
Fungi
Dust
Humidity
Biodiversity
RENOVATION
IN-HOUSE DUST
Biotechnology
Microbial population biology
13. Climate action
QUANTITATIVE PCR ANALYSIS
Housing
HEALTH
business
Culture independent
Research Article
Zdroj: BMC Microbiology
BMC Microbiology, Vol 11, Iss 1, p 235 (2011)
ISSN: 1471-2180
Popis: Background Indoor microbial contamination due to excess moisture is an important contributor to human illness in both residential and occupational settings. However, the census of microorganisms in the indoor environment is limited by the use of selective, culture-based detection techniques. By using clone library sequencing of full-length internal transcribed spacer region combined with quantitative polymerase chain reaction (qPCR) for 69 fungal species or assay groups and cultivation, we have been able to generate a more comprehensive description of the total indoor mycoflora. Using this suite of methods, we assessed the impact of moisture damage on the fungal community composition of settled dust and building material samples (n = 8 and 16, correspondingly). Water-damaged buildings (n = 2) were examined pre- and post- remediation, and compared with undamaged reference buildings (n = 2). Results Culture-dependent and independent methods were consistent in the dominant fungal taxa in dust, but sequencing revealed a five to ten times higher diversity at the genus level than culture or qPCR. Previously unknown, verified fungal phylotypes were detected in dust, accounting for 12% of all diversity. Fungal diversity, especially within classes Dothideomycetes and Agaricomycetes tended to be higher in the water damaged buildings. Fungal phylotypes detected in building materials were present in dust samples, but their proportion of total fungi was similar for damaged and reference buildings. The quantitative correlation between clone library phylotype frequencies and qPCR counts was moderate (r = 0.59, p < 0.01). Conclusions We examined a small number of target buildings and found indications of elevated fungal diversity associated with water damage. Some of the fungi in dust were attributable to building growth, but more information on the material-associated communities is needed in order to understand the dynamics of microbial communities between building structures and dust. The sequencing-based method proved indispensable for describing the true fungal diversity in indoor environments. However, making conclusions concerning the effect of building conditions on building mycobiota using this methodology was complicated by the wide natural diversity in the dust samples, the incomplete knowledge of material-associated fungi fungi and the semiquantitative nature of sequencing based methods.
Databáze: OpenAIRE
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