Coordinate extinction of melanocyte-specific gene expression in hybrid cells
| Autor: | Richard L. Davidson, Thomas P. Powers |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Tyrosinase
Molecular Sequence Data Locus (genetics) Hybrid Cells Melanocyte Biology Microphthalmia Mice Cricetinae Gene expression Tumor Cells Cultured Genetics medicine Animals Microphthalmos Receptors Pituitary Hormone Isomerases Melanoma Gene Membrane Glycoproteins Base Sequence Mesocricetus Monophenol Monooxygenase Pigmentation Proteins Cell Biology General Medicine Fibroblasts medicine.disease Molecular biology Reverse transcriptase Intramolecular Oxidoreductases medicine.anatomical_structure Gene Expression Regulation Melanocytes Oxidoreductases Melanocortin 1 receptor |
| Zdroj: | Somatic Cell and Molecular Genetics. 22:41-56 |
| ISSN: | 1572-9931 0740-7750 |
| DOI: | 10.1007/bf02374375 |
| Popis: | Whole cell hybrids and microcell hybrids between mouse fibroblasts and pigmented Syrian hamster melanoma cells were analyzed for coordinate regulation of melanocyte-specific gene products. Extinction of pigmentation was observed in whole-cell hybrids and in a microcell hybrid containing a single mouse chromosome (mouse chromosome 1). Analysis of melanocyte-specific transcripts using reverse transcription, combined with the polymerase chain reaction (RT-PCR), demonstrated that tyrosinase, TRP-1, TRP-2, and microphthalmia transcripts were all absent in unpigmented whole-cell hybrids and in the monochromosomal unpigmented microcell hybrid. A pigmented subclone of this microcell hybrid, however, re-expressed the tyrosinase, TRP-1, TRP-2, and microphthalmia genes. These data suggest that all of these genes are coordinately extinguished by a single fibroblast locus. Since the only fibroblast chromosome detected in the unpigmented microcell hybrid was mouse chromosome 1, these results also suggest that the extinguisher locus affecting the expression of the tyrosinase, TRP-1, TRP-2, and microphthalmia genes in hybrid cells is located on that mouse chromosome (or on a fragment of another chromosome present in the unpigmented monochromosomal microcell hybrid but undetected in our analyses). In contrast to the results with the melanocyte-specific genes mentioned above, transcripts for the melanocortin 1 receptor gene (MC1R) were present in the monochromosomal unpigmented microcell hybrid (although absent in the whole-cell hybrids). This suggests that regulation of MC1R gene expression is distinct from regulation of the other melanocyte-specific genes. |
| Databáze: | OpenAIRE |
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