Investigation of pulmonary involvement in inflammatory bowel disease in an experimental model of colitis
| Autor: | Altug Senol, Bünyamin Aydın, Recep Sutcu, Yıldıran Songur, I. Metin Ciris, Necla Songur, Oğuzhan Aksu |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Pathology medicine.medical_specialty Inflammatory bowel diseases Asymptomatic Inflammatory bowel disease Gastroenterology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Humans Pulmonary involvement Colitis Rats Wistar Lung Acute colitis Experimental model business.industry Tumor Necrosis Factor-alpha Dextran Sulfate medicine.disease digestive system diseases Experimental colitis Rats Vascular endothelial growth factor Disease Models Animal 030228 respiratory system chemistry Dextran sulphate Trinitrobenzenesulfonic Acid Etiology 030211 gastroenterology & hepatology Original Article medicine.symptom business |
| Zdroj: | The Korean Journal of Internal Medicine |
| ISSN: | 2005-6648 |
| Popis: | Background/Aims: Inflammatory bowel disease (IBD) may also involve various extra-intestinal organs. Clinical studies have found asymptomatic/symptomatic pulmonary involvement in 1% to 6% of patients with IBD. The present study histopathologically investigated pulmonary involvement in an experimental model of colitis in order to demonstrate pulmonary tissue involvement in IBD and to expose potential etiological factors. It also explored the relation between inflammation and tissue concentrations of vascular endothelial growth factor (VEGF) and tumor necrosis factor α (TNF-α). Methods: The study comprised 24 male Wistar albino rats. The rats were divided into four groups of six rats each. Acute colitis was induced in two separate groups using either the dextran sulphate sodium (DSS) or trinitrobenzene sulfonic acid (TNBS) method, while the other two groups were used as controls for each model of colitis. Wallace scoring was used for macroscopic assessment of colitis, and the lungs were histopathologically examined. Concentrations of VEGF and TNF-α in pulmonary tissue were measured by the enzyme-linked immunosorbent assay method. Results: The number of animals that had alveolar hemorrhage was significantly higher in the TNBS induced colitis and DSS-induced colitis groups compared to their own control groups (p = 0.015 and p = 0.015, respectively). VEGF and TNF-α concentrations in pulmonary tissues were significantly increased in both the TNBS colitis and DSS colitis groups compared to their own control groups (p = 0.002 and p = 0.004, respectively; and p = 0.002 and p = 0.002, respectively). Conclusions: The present study demonstrated that significant and serious histopathological changes directly associated with colitis occur in the lungs in IBD. |
| Databáze: | OpenAIRE |
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