gammadelta T-cell clones from intestinal intraepithelial lymphocytes inhibit development of CTL responses ex vivo
| Autor: | Judith A. Kapp, Linda M. Kapp, Jeffrey P. Lake, Kyle C. McKenna |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Cytotoxicity
Immunologic Adoptive cell transfer Receptors Antigen T-Cell alpha-beta T cell Immunology Mice Inbred Strains chemical and pharmacologic phenomena Biology Cell Line Immunophenotyping Mice Antigen T-Lymphocyte Subsets Intestine Small Immune Tolerance medicine Animals Immunology and Allergy Cytotoxic T cell Intestinal Mucosa Immunity Mucosal Cells Cultured Mice Inbred BALB C T-cell receptor Epithelial Cells Receptors Antigen T-Cell gamma-delta hemic and immune systems Original Articles T helper cell Molecular biology Clone Cells stomatognathic diseases CTL medicine.anatomical_structure Lymphocyte Culture Test Mixed CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Immunology. 111:155-164 |
| ISSN: | 1365-2567 0019-2805 |
| Popis: | Oral administration of antigen induces a state of tolerance that is associated with activation of CD8+ T cells that can transfer unresponsiveness to naïve syngeneic hosts. These T cells are not lytic, but they inhibit development of antibody, CD4+ T helper cell, and CD8+ cytotoxic T lymphocyte (CTL) responses upon adoptive transfer into naïve, syngeneic mice. In addition, we have shown that depletion of gammadelta T cells by injection of the anti-delta chain antibody (GL3) down modulates the expression of gammadelta T-cell receptor (TCR) and inhibits the induction of oral tolerance to ovalbumin. Oral administration of antigen also fails to induce tolerance in TCR delta-chain knockout mice suggesting that gammadelta T cells play a critical, active role in tolerance induced by orally administered antigen. To further study the contribution of gammadelta T cells to tolerance, murine gammadelta T cells were isolated from intraepithelial lymphocytes (IEL) of the small intestine by stimulation with splenic filler cells, concanavalin A and growth factors. gammadelta IEL lines demonstrated lytic activity in a redirected lysis assay. gammadelta T-cell clones express different gammadelta TCR genes and secrete large amounts of interleukin (IL)-10, but little or no IL-2, IL-4, or interferon-gamma. gammadelta IEL clones expressed transforming growth factor-beta1 and macrophage migration inhibitory factor, as well as IL-10, mRNA. Moreover, gammadelta T-cell clones potently inhibited the generation of CTL responses by secreted molecules rather than by direct cell-to-cell contact. |
| Databáze: | OpenAIRE |
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