The AraC Negative Regulator family modulates the activity of histone-like proteins in pathogenic bacteria
| Autor: | Melissa M. Kendall, A. Brooke Sauder, Araceli E. Santiago, Michael B. Yan, Mario Meza-Segura, James P. Nataro, Fernando Ruiz-Perez, David A. Rasko, Tracy H. Hazen |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
AraC Transcription Factor Cell Membranes Gene Expression Electrophoretic Mobility Shift Assay Yersinia Restriction Fragment Mapping Pathology and Laboratory Medicine Polymerase Chain Reaction Biochemistry Transcriptome Histones Enteropathogenic Escherichia coli Mice Gene expression Transcriptional regulation Medicine and Health Sciences lcsh:QH301-705.5 Escherichia coli Infections Genetics Regulation of gene expression Mice Inbred BALB C Virulence Escherichia coli Proteins Bacterial Pathogens Medical Microbiology Cellular Structures and Organelles Pathogens Research Article lcsh:Immunologic diseases. Allergy 030106 microbiology Immunology Biology Microbiology 03 medical and health sciences Extraction techniques Gene Types Virology DNA-binding proteins Gene silencing Animals Electrophoretic mobility shift assay Gene Regulation Molecular Biology Techniques Gene Molecular Biology Microbial Pathogens Biology and life sciences Gene Mapping Proteins Membrane Proteins Gene Expression Regulation Bacterial Cell Biology biology.organism_classification Outer Membrane Proteins RNA extraction Research and analysis methods lcsh:Biology (General) Regulator Genes Parasitology lcsh:RC581-607 |
| Zdroj: | PLoS Pathogens PLoS Pathogens, Vol 13, Iss 8, p e1006545 (2017) |
| ISSN: | 1553-7374 |
| Popis: | The AraC Negative Regulators (ANR) comprise a large family of virulence regulators distributed among diverse clinically important Gram-negative pathogens, including Vibrio spp., Salmonella spp., Shigella spp., Yersinia spp., Citrobacter spp., and pathogenic E. coli strains. We have previously reported broad effects of the ANR members on regulators of the AraC/XylS family. Here, we interrogate possible broader effects of the ANR members on the bacterial transcriptome. Our studies focused on Aar (AggR-activated regulator), an ANR family archetype in enteroaggregative E. coli (EAEC) isolate 042. Transcriptome analysis of EAEC strain 042, 042aar and 042aar(pAar) identified more than 200 genes that were differentially expressed (+/- 1.5 fold, p Author summary The AraC Negative Regulators (ANR) is a large family of negative regulators distributed in several clinically relevant Gram-negative pathogens, including Vibrio spp., Salmonella spp., Shigella spp., Yersinia spp., Citrobacter spp., pathogenic E. coli, and members of the Pasteurellaceae. Previously, we showed that the ANR family suppresses transcriptional expression of virulence factors such as fimbriae, toxins, and the type VI secretion system by directly down-regulating AraC/XylS master regulators. Transcriptome and biochemical analysis of Aar (AggR-activated regulator), an ANR family archetype in enteroaggregative E. coli (EAEC) 042, demonstrated that Aar binds directly to H-NS and modulates the H-NS-induced gene expression. Accordingly, mutation of aar decreased expression of the H-NS-regulated Lpf fimbriae, LPS-related enzymes, GadXW operon and porins. Importantly, Aar was highly expressed in the mouse intestinal tract and was found to be necessary for maximal H-NS expression. These findings unveil an exquisite regulatory network in pathogenic bacteria, which operates by concomitant control of master transcriptional regulators of the AraC family and global negative H-NS regulators. |
| Databáze: | OpenAIRE |
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