Primary cilium‐dependent mechanosensing is mediated by adenylyl cyclase 6 and cyclic AMP in bone cells
| Autor: | Sara Temiyasathit, Padmaja Tummala, Clarence C. Quah, Christopher R. Jacobs, Ronald Y. Kwon |
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| Rok vydání: | 2010 |
| Předmět: |
Gadolinium
Biology Mechanotransduction Cellular Osteocytes Biochemistry ADCY10 Research Communications Adenylyl cyclase Mice chemistry.chemical_compound Bone cell Cyclic AMP Genetics Animals Cilia Mechanotransduction Molecular Biology Cilium ADCY9 Membrane Transport Proteins Cell biology Perfusion chemistry Signal transduction Intracellular Adenylyl Cyclases Biotechnology |
| Zdroj: | The FASEB Journal. 24:2859-2868 |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fj.09-148007 |
| Popis: | Primary cilia are chemosensing and mechanosensing organelles that regulate remarkably diverse processes in a variety of cells. We previously showed that primary cilia play a role in mediating mechanosensing in bone cells through an unknown mechanism that does not involve extracellular Ca2+-dependent intracellular Ca2+ release, which has been implicated in all other cells that transduce mechanical signals via the cilium. Here, we identify a molecular mechanism linking primary cilia and bone cell mechanotransduction that involves adenylyl cyclase 6 (AC6) and cAMP. Intracellular cAMP was quantified in MLO-Y4 cells exposed to dynamic flow, and AC6 and primary cilia were inhibited using RNA interference. When exposed to flow, cells rapidly ( |
| Databáze: | OpenAIRE |
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