Correlation of invasion and metastasis of cancer cells, and expression of the RAD21 gene in oral squamous cell carcinoma
| Autor: | Tetsuhiko Tachikawa, Tadateru Aida, Gou Yamamoto, Yuuki Nagoshi, Tarou Irie, Reiko Tsuchiya |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Cell Survival Mice Nude Apoptosis Cell Cycle Proteins Biology Hydroxamic Acids Pathology and Forensic Medicine Metastasis Mice Cell Line Tumor Carcinoma medicine Animals Humans Neoplasm Invasiveness RNA Messenger Molecular Biology Aged Laser capture microdissection Aged 80 and over Regulation of gene expression Reverse Transcriptase Polymerase Chain Reaction Nuclear Proteins Cancer Cell Biology General Medicine Middle Aged Phosphoproteins medicine.disease DNA-Binding Proteins Gene Expression Regulation Neoplastic Cell culture Cancer cell Carcinoma Squamous Cell Dactinomycin Cancer research Female Mouth Neoplasms |
| Zdroj: | Virchows Archiv. 448:435-441 |
| ISSN: | 1432-2307 0945-6317 |
| Popis: | Although RAD21 is involved in the repair of double-strand breaks in DNA and is essential for mitotic growth, its role in cancer has been unclear. In this study, the relevance of RAD21 gene expression to the invasion and metastasis of oral squamous cell carcinoma was clarified using laser microdissection and real-time polymerase chain reaction (PCR). Using two different metastatic potential oral squamous cells [high-metastatic-potential squamous cell carcinoma cells (SAS-Ly) and low-metastatic-potential squamous cell carcinoma cells (SAS)], the relation of RAD21 gene expression to apoptosis, invasion, and metastasis was examined. The results showed that RAD21 gene expression was significantly decreased in oral squamous cell carcinoma when it expressed the INFbeta and INFgamma invasion patterns in comparison with the INFalpha invasion pattern (p0.01). In addition, in comparison with SAS cells, SAS-Ly cells indicated tolerance to cell death induced by an apoptosis induction reagent, while the expression level of the RAD21 gene in SAS cells was increased by the apoptosis induction reagent. However, in SAS-Ly cells, the reagent induced no significant difference. Our findings indicate that the RAD21 gene was closely related to the invasion and metastasis of cancer cells. |
| Databáze: | OpenAIRE |
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