T‐705‐modified ssRNA in complex with Lassa virus nucleoprotein exhibits nucleotide splaying and increased water influx into the RNA‐binding pocket

Autor:	Ayodeji A. Ojo, Olaposi I Omotuyi, Niyi S. Adelakun, Tomisin Happy Ogunwa, David Safronetz, Oyekanmi Nash
Rok vydání:	2019
Předmět:	Static Electricity Molecular Dynamics Simulation medicine.disease_cause 01 natural sciences Biochemistry Virus chemistry.chemical_compound Drug Discovery medicine Nucleotide Ribonuclease Lassa virus Pharmacology chemistry.chemical_classification Binding Sites biology Nucleotides 010405 organic chemistry Ribavirin Organic Chemistry Water RNA Hydrogen Bonding Glycosidic bond 0104 chemical sciences Nucleoprotein 010404 medicinal & biomolecular chemistry Nucleoproteins chemistry biology.protein Biophysics Nucleic Acid Conformation Molecular Medicine
Zdroj:	Chemical Biology & Drug Design. 93:544-555
ISSN:	1747-0285 1747-0277
Popis:	Lassa virus infection is clinically characterized by multiorgan failure in humans. Without an FDA-approved vaccine, ribavirin is the frontline drug for the treatment but with attendant toxicities. 6-Fluoro-3-hydroxy-2-pyrazinecarboxamide (T-705) is an emerging alternative drug with proven anti-Lassa virus activity in experimental model. One of the mechanisms of action is its incorporation into nascent single-strand RNA (ssRNA) which forms complex with Lassa nucleoprotein (LASV-NP). Here, using molecular dynamics simulation, the structural and electrostatics changes associated with LASV-NP-ssRNA complex have been studied when none, one, or four of its bases has been substituted with T-705. The results demonstrated that glycosidic torsion angle χ (O4'-C1'-N1-C2) rotated from high-anti- (-110° and -60°) to the syn- conformation (+30) with increased T-705 substitution. Similarly, increased T-705 substitution resulted in increased splaying (55°-70°), loss of ssRNA-LASV-NP H-bond interaction, increased water influx into the ssRNA-binding pocket, and decreased electrostatic potentials of ssRNA pocket. Furthermore, strong positively correlated motion observed between α6 residues (aa: 128-145) and its contact ssRNA bases (5-7) is weakened in Apo biosystem and transitioned into anticorrelated motions in ssRNA-bound LASV-NP biosystem. Finally, LASV genome may become more accessible to cellular ribonuclease access with T-705 incorporation due to loss of NP interaction.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::890bdc11fc672588a07fb26cf920ad55 https://doi.org/10.1111/cbdd.13451 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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