| Autor: |
Jing, Xiaoyan, Gong, Yanhai, Xu, Teng, Davison, Paul A., MacGregor-Chatwin, Craig, Hunter, C. Neil, Xu, La, Meng, Yu, Ji, Yuetong, Ma, Bo, Xu, Jian, Huang, Wei E. |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
BioDesign Research. 2022 |
| ISSN: |
2693-1257 |
| DOI: |
10.34133/2022/9782712 |
| Popis: |
The majority of marine microbes remain uncultured, which hinders the identification and mining of CO 2 -fixing genes, pathways, and chassis from the oceans. Here, we investigated CO 2 -fixing microbes in seawater from the euphotic zone of the Yellow Sea of China by detecting and tracking their 13 C-bicarbonate ( 13 C-HCO 3 - ) intake via single-cell Raman spectra (SCRS) analysis. The target cells were then isolated by Raman-activated Gravity-driven Encapsulation (RAGE), and their genomes were amplified and sequenced at one-cell resolution. The single-cell metabolism, phenotype and genome are consistent. We identified a not-yet-cultured Pelagibacter spp., which actively assimilates 13 C-HCO 3 - , and also possesses most of the genes encoding enzymes of the Calvin-Benson cycle for CO 2 fixation, a complete gene set for a rhodopsin-based light-harvesting system, and the full genes necessary for carotenoid synthesis. The four proteorhodopsin (PR) genes identified in the Pelagibacter spp. were confirmed by heterologous expression in E. coli . These results suggest that hitherto uncultured Pelagibacter spp. uses light-powered metabolism to contribute to global carbon cycling. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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