A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts
| Autor: | Ping Li, Douglas Bowman, Helen He, Zhongmin Xiang, Zhi Li, Lunyin Yu, Natalie Roy D’Amore, James E. Brownell, Jie Yu, Jouhara Chouitar, Stephen Tirrell, Matthew Theisen, Yu Yang |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Article Subject Clinical Biochemistry Immunology Autophagy Formalin fixed Biology Molecular biology Paraffin embedded Human tumor 03 medical and health sciences 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Immunohistochemistry Signal amplification Research Article |
| Zdroj: | Journal of Biomarkers |
| ISSN: | 2090-7699 2090-8660 |
| Popis: | Objective. Use of tyramide signal amplification (TSA) to detect autophagy biomarkers in formalin fixed and paraffin embedded (FFPE) xenograft tissue. Materials and Methods. Autophagy marker regulation was studied in xenograft tissues using Amp HQ IHC and standard IHC methods. Results. The data demonstrate the feasibility of using high sensitivity TSA IHC assays to measure low abundant autophagy markers in FFPE xenograft tissue. |
| Databáze: | OpenAIRE |
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