Remyelination of Demyelinated CNS Axons by Transplanted Human Schwann Cells: The Deleterious Effect of Contaminating Fibroblasts
| Autor: | C. M. H. Brierley, Yasushi Iwashita, Jennifer M. Gilson, D. A. S. Compston, A. J. Crang, Neil J Scolding, William F. Blakemore |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Cell Biomedical Engineering lcsh:Medicine Schwann cell Cell Separation Biology Nerve Fibers Myelinated Rats Nude 03 medical and health sciences 0302 clinical medicine medicine Animals Humans Autologous transplantation Remyelination Fibroblast Cells Cultured Transplantation Multiple sclerosis lcsh:R Cell Biology Fibroblasts Middle Aged medicine.disease Nerve Regeneration Rats Microscopy Electron 030104 developmental biology medicine.anatomical_structure Spinal Cord nervous system Peripheral nervous system biology.protein Schwann Cells Antibody 030217 neurology & neurosurgery |
| Zdroj: | Cell Transplantation, Vol 10 (2001) |
| ISSN: | 1555-3892 |
| Popis: | Areas of demyelination can be remyelinated by transplanting myelin-forming cells. Schwann cells are the naturally remyelinating cells of the peripheral nervous system and have a number of features that may make them attractive for cell implantation therapies in multiple sclerosis, in which spontaneous but limited Schwann cell remyelination has been well documented. Schwann cells can be expanded in vitro, potentially affording the opportunity of autologous transplantation; and they might also be spared the demyelinating process in multiple sclerosis. Although rat, cat, and monkey Schwann cells have been transplanted into rodent demyelinating lesions, the behavior of transplanted human Schwann cells has not been evaluated. In this study we examined the consequences of injecting human Schwann cells into areas of acute demyelination in the spinal cords of adult rats. We found that transplants containing significant fibroblast contamination resulted in deposition of large amounts of collagen and extensive axonal degeneration. However, Schwann cell preparations that had been purified by positive immunoselection using antibodies to human low-affinity nerve growth factor receptor containing less than 10% fibroblasts were associated with remyelination. This result indicates that fibroblast contamination of human Schwann cells represents a greater problem than would have been appreciated from previous studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|