Design and Implementation of a High-Throughput Vibrating Module for Nucleic Acid Detection System
| Autor: | Zhu Chen, Hui Chen, Chao Wang, Tonghua Liu, Song Li, He Zhu, Nongyue He, Yanping Huang, Zunliang Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Signal processing
Temperature control Materials science Lysis Elution Extraction (chemistry) Temperature Biomedical Engineering Nucleic acid methods Bioengineering General Chemistry Condensed Matter Physics Magnetics Nucleic Acids Nucleic acid Magnetic nanoparticles General Materials Science Biological system Nucleic Acid Amplification Techniques |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 20:2165-2170 |
| ISSN: | 1533-4880 |
| Popis: | The high-throughput nucleic acid detection system provides a good solution for detecting nucleic acids more safely, rapidly and accurately, which greatly improves the detection efficiency. Highthroughput nucleic acid detection mainly includes three steps: signal acquisition, signal amplification and signal processing. Therefore, obtaining the purified nucleic acid is the primary task of the nucleic acid detection, and the quality of the nucleic acid has a significant impact on results. In this paper, we employed the magnetic nanoparticle technology for extracting nucleic acids based on the platform of large liquid handling workstation and designed a matching vibrating module. The involved steps of core method, magnetic bead nucleic acid extraction technology, are mainly concerned with the cell lysis, nucleic acid binding, nucleic acid purification and magnetic particles elution. During the extraction process, specific temperature is required for the lysis and elution. It was shown that the temperature control part designed in this paper has the reliable stability, high accuracy by using the incremental proportion-integration-differentiation (PID) algorithm, with the control accuracy up to ±0.5 °C. The temperature regulating time is about 90 s, which can meet the experimental requirements. Besides, the vibrating uniformity of this module was further verified by protein concentration test, which proved that the module has the excellent performance and can be consistent with the experimental indictors of the nucleic acid extraction. |
| Databáze: | OpenAIRE |
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