Adhesive interactions between milk fat globule membrane and Lactobacillus rhamnosus GG inhibit bacterial attachment to Caco-2 TC7 intestinal cell

Autor: Justine Guerin, Faustine Gomand, Marion Huguet, Jennifer Burgain, Grégory Francius, Claire Soligot, Joël Scher, Frédéric Borges, Sofiane El-Kirat-Chatel, Claire Gaiani, Yves Le Roux, Sarah Lebeer
Přispěvatelé: Laboratoire d'Ingénierie des Biomolécules (LIBio), Université de Lorraine (UL), Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA), Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Department of Bioscience Engineering, Universiteit Antwerpen [Antwerpen], Centre of Microbial and Plant Genetics, Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Université de Lorraine (UL)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
030106 microbiology
Matrix (biology)
Microscopy
Atomic Force
Pilus
Bacterial Adhesion
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Colloid and Surface Chemistry
Lactobacillus rhamnosus
Animals
Humans
[CHIM]Chemical Sciences
Physical and Theoretical Chemistry
Cell adhesion
Biology
ComputingMilieux_MISCELLANEOUS
[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Glycoproteins
biology
Lacticaseibacillus rhamnosus
Physics
Wild type
food and beverages
Surfaces and Interfaces
General Medicine
Adhesion
Lipid Droplets
[CHIM.MATE]Chemical Sciences/Material chemistry
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Lactic acid
Chemistry
030104 developmental biology
Enterocytes
chemistry
Fimbriae
Bacterial
Mutation
Caco-2 Cells
Glycolipids
Bacteria
Biotechnology
Zdroj: Colloids and Surfaces B: Biointerfaces
Colloids and Surfaces B: Biointerfaces, Elsevier, 2018, 167, pp.44-53. ⟨10.1016/j.colsurfb.2018.03.044⟩
Colloids and surfaces: B : biointerfaces
ISSN: 0927-7765
DOI: 10.1016/j.colsurfb.2018.03.044⟩
Popis: Milk is the most popular matrix for the delivery of lactic acid bacteria, but little is known about how milk impacts bacterial functionality. Here, the adhesion mechanisms of Lactobacillus rhamnosus GG (LGG) surface mutants to a milk component, the milk fat globule membrane (MFGM), were compared using atomic force microscopy (AFM). AFM results revealed the key adhesive role of the LGG SpaCBA pilus in relation to MFGM. A LGG mutant without exopolysaccharides but with highly exposed pili improved the number of adhesive events between LGG and MFGM compared to LGG wild type (WT). In contrast, the number of adhesive events decreased significantly for a LGG mutant without SpaCBA pill. Moreover, the presence of MFGM in the dairy matrix was found to decrease significantly the bacterial attachment ability to Caco-2 TC7 cells. This work thus demonstrated a possible competition between LGG adhesion to MFGM and to epithelial intestinal cells. This competition could negatively impact the adhesion capacity of LGG to intestinal cells in vivo, but requires further substantiation. (C) 2018 Elsevier B.V. All rights reserved.
Databáze: OpenAIRE
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