The mitogaligin protein is addressed to the nucleus via a non-classical localization signal
| Autor: | Fabienne Brulé, Stéphane Charpentier, Lucile Mollet, Thierry Normand, Martine Dubois, Alain Legrand, Patrick Gonzalez, Pauline Robinet |
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| Přispěvatelé: | Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut de Mathématiques de Bordeaux (IMB), Université Bordeaux Segalen - Bordeaux 2-Université Sciences et Technologies - Bordeaux 1-Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux (Bordeaux INP)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2009 |
| Předmět: |
Galectins
Molecular Sequence Data Nuclear Localization Signals Biophysics Active Transport Cell Nucleus Importin CELL-DEATH PROTEIN Mitochondrion Biology medicine.disease_cause SEQUENCE Biochemistry 03 medical and health sciences Dual localization MITOCHONDRIA Protein targeting medicine Humans Mitogaligin Amino Acid Sequence Molecular Biology Gene 030304 developmental biology HSPA9 Sequence Deletion Cell Nucleus 0303 health sciences MOLECULAR-CLONING IDENTIFICATION Cytochrome c 030302 biochemistry & molecular biology INTRACELLULAR-LOCALIZATION Cell Biology Blood Proteins Unconventional nuclear localization signal GENE TRANSLOCATION Cell biology Protein Structure Tertiary Cytosol medicine.anatomical_structure biology.protein VIRUS IMPORTIN Nucleus HeLa Cells |
| Zdroj: | Biochemical and Biophysical Research Communications Biochemical and Biophysical Research Communications, Elsevier, 2010, 392 (1), pp.53-57. ⟨10.1016/j.bbrc.2009.12.162⟩ |
| ISSN: | 1090-2104 0006-291X |
| DOI: | 10.1016/j.bbrc.2009.12.162⟩ |
| Popis: | International audience; Mitogaligin, a protein encoded by galig, an internal cytotoxic gene of the galectin-3 locus, is mostly a mitochondrial protein. Mitochondrial targeting is due to an already identified mitochondrial localization signal. Interaction of mitogaligin with mitochondria leads to cytochrome c cytosolic leakage and ultimately to cell death. We have previously pointed out that mitogaligin can also be directed to the nucleus when the mitochondrial addressing signal is inactivated, indicating a possible dual intracellular localization of the protein. When expressed in the nucleus, mitogaligin exhibits also apoptotic properties leading to cell death. In this report, we show that nuclear addressing of mitogaligin depends on a sequence differing from classical signals containing basic, lysine or proline-tyrosine rich residues. The signal consists of a long sequence of amino acids residues based on a series of a short repetitive degenerated sequence. |
| Databáze: | OpenAIRE |
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