Endothelial modulation of a nitric oxide donor complex-induced relaxation in normotensive and spontaneously hypertensive rats

Autor: Simone R. Potje, Marcella D. Grando, Jéssica A. Troiano, Lusiane Maria Bendhack, Cristina Antoniali, Roberto Santana da Silva, Murilo E. Graton
Přispěvatelé: Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP)
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Muscle Relaxation
VASODILATAÇÃO
Vasodilation
030204 cardiovascular system & hematology
TERPY
SHR
chemistry.chemical_compound
0302 clinical medicine
Coordination Complexes
Enos
Rats
Inbred SHR
General Pharmacology
Toxicology and Pharmaceutics
Mesenteric arteries
Oxadiazoles
biology
Chemistry
Mesenteric artery
General Medicine
Potassium channel
Mesenteric Arteries
NG-Nitroarginine Methyl Ester
medicine.anatomical_structure
eNOS
cardiovascular system
Nitric oxide donor
medicine.medical_specialty
Nitric Oxide Synthase Type III
Endothelium
Ruthenium
General Biochemistry
Genetics and Molecular Biology
Nitric oxide
03 medical and health sciences
Quinoxalines
Internal medicine
Potassium Channel Blockers
medicine
Animals
Nitric Oxide Donors
Tetraethylammonium
Dose-Response Relationship
Drug
biology.organism_classification
Rats
030104 developmental biology
Endocrinology
Guanylate Cyclase
Vascular Resistance
Endothelium
Vascular
Soluble guanylyl cyclase
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 0024-3205
DOI: 10.1016/j.lfs.2018.03.055
Popis: Made available in DSpace on 2018-12-11T17:24:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-05-15 We hypothesized that endothelium modulates relaxation induced by a nitric oxide (NO) donor ruthenium complex (TERPY, [Ru(terpy)(bdq)NO]3+) in mesenteric arteries of normotensive and spontaneously hypertensive (SHR) rats in different ways. We analyzed the mechanism involved in TERPY-induced relaxation in the second and third branches of mesenteric arteries and investigated how endothelium contributes to the TERPY vasodilator effect on SHR blood vessels. TERPY induced concentration-dependent relaxation in endothelium-denuded (E-) and endothelium-intact (E+) mesenteric arteries of normotensive rats and SHR. Pretreatment with ODQ (which inhibits soluble guanylyl cyclase) or TEA (tetraethylammonium, which blocks potassium channels) significantly reduced the TERPY vasodilator effect on E- mesenteric arteries of normotensive rats and SHR. The presence of endothelium shifted the concentration-effect curves for TERPY in E+ mesenteric arteries of normotensive rats to the right. Conversely, the presence of endothelium shifted the concentration-effect curves for TERPY in the case of SHR E+ mesenteric arteries to the left, which suggested increased potency. L-NNA, a more selective endothelial NO synthase (eNOS) inhibitor, reduced TERPY potency in SHR. The presence of endothelium and notably of NOS contributed to the TERPY vasodilator action in SHR: TERPY promoted eNOS Ser1177 phosphorylation with consequent NO production and increased soluble guanylyl cyclase activity, which may have directly activated potassium channels. Programa Multicêntrico de Pós-graduação em Ciências Fisiológicas - SBFis, São Paulo State University (UNESP), School of Dentistry, Araçatuba, Department of Basic Sciences, Brazil University of São Paulo (USP), Faculty of Pharmaceutical Sciences of Ribeirão Preto, Department of Physics and Chemistry, Brazil Programa Multicêntrico de Pós-graduação em Ciências Fisiológicas - SBFis, São Paulo State University (UNESP), School of Dentistry, Araçatuba, Department of Basic Sciences, Brazil. Electronic address: crisant@foa.unesp.br
Databáze: OpenAIRE
Externí odkaz: