Glycolytic Proteins Interact With Intracellular Melatonin in Saccharomyces cerevisiae
| Autor: | Albert Mas, María Ángeles Morcillo-Parra, Gemma Beltran, María Jesús Torija, Maria José Valera |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Cell signaling endocrine system fermented beverages Saccharomyces cerevisiae lcsh:QR1-502 melatonin Mitochondrion GADPH Microbiology lcsh:Microbiology pyruvate kinase Melatonin 03 medical and health sciences Extracellular medicine 030304 developmental biology Original Research 0303 health sciences Molecular mass biology 030306 microbiology Chemistry glycolysis biology.organism_classification Yeast enolase Biochemistry Intracellular hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 10 (2019) |
| ISSN: | 1664-302X |
| Popis: | Melatonin is a bioactive compound that is present in fermented beverages and synthesized by yeast during alcoholic fermentation. Many studies have shown that melatonin interacts with some mammalian proteins, such as sirtuins or orphan receptor family proteins. The aim of this study was to determine the intracellular synthesis profile of melatonin in Saccharomyces cerevisiae and to identify the proteins that may interact with this molecule in yeast cells. Melatonin from fermentation samples was analyzed by liquid chromatography mass spectrometry, and proteins bound to melatonin were immunopurified by melatonin-IgG-Dynabeads. Melatonin was produced intracellularly in the lag phase of yeast growth and was exported to the extracellular media during the stationary phase. During this period, melatonin was bound to six proteins with molecular weights from 55 to 35 kDa. Sequence analysis showed that most proteins shared high levels of homology with glycolytic enzymes. An RNA-binding protein was also identified, the elongation alpha factor, which is related to mitochondria. This study reports for the first time the interaction of melatonin and proteins inside yeast cells. These results highlight the possible role of melatonin as a signal molecule and provide a new perspective for understanding its role in yeast. |
| Databáze: | OpenAIRE |
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