High levels of soluble CTLA-4 are present in anti-mitochondrial antibody positive, but not in antibody negative patients with primary biliary cirrhosis
| Autor: | Brunetta Porcelli, Ignazio Brusca, Nicola Bizzaro, Princey Antola, Maria Alessio, Danilo Villalta, Marilina Tampoia, Elio Tonutti, Renato Tozzoli, Daniele Saverino, Giampaola Pesce, Marcello Bagnasco |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Bilirubin Recombinant Fusion Proteins Immunology lcsh:Medicine Autoantigens Autoimmune Diseases chemistry.chemical_compound Primary biliary cirrhosis Western blot Antigen parasitic diseases medicine Medicine and Health Sciences Humans CTLA-4 Antigen lcsh:Science Autoantibodies Multidisciplinary Primary Biliary Cirrhosis medicine.diagnostic_test business.industry Liver Cirrhosis Biliary lcsh:R Autoantibody Case-control study Biology and Life Sciences Antigens Nuclear Middle Aged medicine.disease Mitochondria Up-Regulation Nuclear Pore Complex Proteins chemistry CTLA-4 Case-Control Studies lcsh:Q Female Clinical Immunology business Anti-mitochondrial antibody Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 11, p e112509 (2014) |
| ISSN: | 1932-6203 |
| Popis: | Primary biliary cirrhosis (PBC) is a chronic autoimmune cholestatic liver disease frequently characterized by anti-mitochondrial autoantibodies (AMA). A minority of patients are AMA-negative. Cytotoxic-T-Lymphocyte-Antigen-4 (CTLA-4) is a surface molecule expressed on activated T-cells delivering a critical negative immunoregulatory signal. A soluble form of CTLA-4 (sCTLA-4) has been detected at high concentrations in several autoimmune diseases, and its possible functional meaning has been suggested. We aimed to evaluate sCTLA-4 concentration in sera of patients with PBC and to correlate it to immunological abnormalities associated with the disease. Blood samples were collected from 82 PBC-patients diagnosed according to international criteria (44 AMA-positive/MIT3-positive and 38 AMA-negative-MIT3-negative), and 65 controls. sCTLA-4 levels were evaluated by ELISA and Western blot. Increased sCTLA-4 concentrations were found in all AMA-positive PBC-patients, but in none of the AMA-negative ones, nor in normal controls or in controls with unrelated liver diseases. sCTLA-4 presence was associated with autoantibodies against MIT3, but not with nuclear autoantibodies (sp100, gp210). This is the first study to demonstrate that levels of sCTLA-4 are elevated in sera of PBC patients. However, they are clearly restricted to patients with AMA positivity, suggesting an immunological difference with respect to AMA-negative ones. |
| Databáze: | OpenAIRE |
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