Reliability and Role of Mutation-specific H3F3A (Histone 3-3) G34W Immunohistochemistry to Differentiate Giant Cell Tumor of Bone From its Clinicoradiologic and Histologic Mimics: An Institutional Study
| Autor: | Himanshu Rohela, Anila Sharma, Meenakshi Kamboj, Ankush Jajodia, Sunil Pasricha, Gurudutt Gupta, Akshay Tiwari, Anurag Mehta, Garima Durga, Manish Pruthi, Ankur Kumar, Venkata Pradeep Babu Koyyala |
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| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty Histology Bone Neoplasms medicine.disease_cause Pathology and Forensic Medicine Histones medicine Neoplasm Humans Malignant Giant Cell Tumor Giant Cell Tumor of Bone Mutation biology business.industry Reproducibility of Results medicine.disease Predictive value Immunohistochemistry Medical Laboratory Technology Histone biology.protein Antibody business Giant-cell tumor of bone |
| Zdroj: | Applied immunohistochemistrymolecular morphology : AIMM. 30(1) |
| ISSN: | 1533-4058 |
| Popis: | Giant cell tumor of bone (GCTB) is a benign neoplasm, which can sometimes be a diagnostic challenge, especially in small biopsies, due to its histologic benign and malignant mimics. We evaluated the role of H3.3 G34W immunohistochemistry (IHC) antibody in diagnosing GCTB and its role in differentiating it from its close histologic mimics. A total of 120 cases (80 cases of GCTB and 40 cases of histologic mimics) were retrieved and subjected to IHC. Of 80 cases of GCTB, 72 cases showed a positive nuclear immunoexpression, while all 40 cases of histologic mimics of GCTB showed a negative staining for H3.3 G34W IHC. Sensitivity and specificity of this mutation-specific antibody for diagnosis of GCTB was 90% and 100%, respectively, while, the positive predictive value and the negative predictive value were 100% and 83.3%, respectively. A positive expression of H3.3 G34W was seen in all 5 cases of GCTB, postdenosumab therapy, as well as, in all 3 cases of malignant giant cell tumor. The presented study showed that H3.3 G34W mutation-specific IHC is a reliable and specific marker for GCTB and can help distinguish it from the histologic mimics due to distinct therapeutic implications. |
| Databáze: | OpenAIRE |
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