Tetanus toxoid-pulsed monocyte vaccination for augmentation of collateral vessel growth
| Autor: | Joerg Herold, Alexander Francke, Soenke Weinert, Burkhart Schraven, Ruediger C. Braun-Dullaeus, Alexander Schmeisser, Katrin Hebel, Friedich‐Wilhelm Roehl, Ruth H. Strasser |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Time Factors T-Lymphocytes Collateral Circulation Neovascularization Physiologic Hindlimb Adaptive Immunity Vascular Medicine Monocytes Mice Immune system Ischemia Internal medicine medicine Tetanus Toxoid Animals Muscle Skeletal cell transplantation Cells Cultured Original Research Mice Inbred BALB C business.industry Monocyte Toxoid Recovery of Function Acquired immune system Coculture Techniques Immunity Innate innate and adaptive immune system Transplantation Mice Inbred C57BL Disease Models Animal Endocrinology medicine.anatomical_structure Regional Blood Flow arteriogenesis Immunology monocyte Cytokines Immunization Arteriogenesis Cardiology and Cardiovascular Medicine business Ex vivo |
| Zdroj: | Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease |
| ISSN: | 2047-9980 |
| Popis: | Background The pathogenesis of collateral growth (arteriogenesis) has been linked to both the innate and adaptive immune systems. While therapeutic approaches for the augmentation of arteriogenesis have focused on innate immunity, exploiting both innate and adaptive immune responses has not been examined. We hypothesized that tetanus toxoid (tt) immunization of mice followed by transplantation of monocytes (Mo) exposed ex vivo to tt augments arteriogenesis after ligation of the hind limb. Methods and Results Mo were generated from nonimmunized BALB /c mice, exposed ex vivo to tt for 24 hours and intravenously injected (ttMo, 2.5×10 6 ) into the tail veins of tt‐immunized syngeneic mice whose hind limbs had been ligated 24 hours prior to transplantation. Laser Doppler perfusion imaging was applied, and a perfusion index ( PI ) was calculated (ratio ligated/unligated). Twenty‐one days after ligation, the arteriogenesis of untreated BALB /c mice was limited ( PI =0.49±0.09). Hind limb function was impaired in 80% of animals. Injection of non‐engineered Mo insignificantly increased the PI to 0.56±0.07. However, ttMo transplantation resulted in a strong increase of the PI to 0.82±0.08 (n=7; P CD 4‐antibody pretreatment ( PI =0.50±0.08; n=17; P Conclusions Transplantation of ttMo into pre‐immunized mice strongly promotes arteriogenesis. This therapeutic approach is feasible and highly attractive for the alleviation of morbidity associated with vascular occlusive disease. |
| Databáze: | OpenAIRE |
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