Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro
| Autor: | Kellen Lagares Ferreira Silva, Sidney Pereira de Oliveira, Rogério Mamoru Suzuki, Wagner de Melo Ferreira, Jack Wild Pereira Soares Júnior |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
culture media
0106 biological sciences meios de cultura histological study orquídea Plant Science Horticulture 01 natural sciences Acclimatization lcsh:Botany growth regulators estudo histológico orchid lcsh:QH301-705.5 Root formation Orchidaceae biology Catasetum macrocarpum 04 agricultural and veterinary sciences biology.organism_classification In vitro lcsh:QK1-989 acclimatization lcsh:Biology (General) Germination Seedling aclimatização Shoot 040103 agronomy & agriculture 0401 agriculture forestry and fisheries reguladores de crescimento 010606 plant biology & botany |
| Zdroj: | Rodriguésia v.69 n.4 2018 Rodriguésia Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ) instacron:JBRJ Rodriguésia, Vol 69, Iss 4, Pp 2137-2151 |
| ISSN: | 2175-7860 0370-6583 |
| Popis: | Catasetum macrocarpum is an epiphytic orchid that has been subjected to strong environmental pressure in the state of Tocantins. This investigation aimed at studying the germination, growth and morpho-anatomical development of C. macrocarpum under in vitro conditions. The effects of three culture media on the in vitro germination and on the multiplication and growth of 90-day-old seedlings were studied: Murashige & Skoog (full- and half-strength), Knudson C, and Vacin & Went. The effects of different concentrations of benzyladenine (BA) and naphthaleneacetic acid (NAA) on the multiplication and growth of 120-day-old plants were evaluated. Anatomical studies were conducted on protocorms at different developmental stages. Acclimatization was also carried out. Knudson C was the best medium for seed germination whereas Vacin & Went promoted the greatest protocorm development. Half-strength Murashige & Skoog was the most effective medium for seedling multiplication and growth. The results revealed that 1 mg L-1 BA was the best treatment for shoot proliferation and leaf production. NAA at 0.5 mg L-1 strongly favored root formation. The anatomical study revealed that the early stages of C. macrocarpum development do not always coincide with the morphological phases described. The acclimatization of C. macrocarpum plants provided successful results regarding plant survival. |
| Databáze: | OpenAIRE |
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