Detection of Chronic bee paralysis virus (CBPV) genome and its replicative RNA form in various hosts and possible ways of spread

Autor: Jean-Paul Faucon, Violaine Olivier, Magali Ribière, Nicolas Cougoule, Philippe Blanchard, Frank M. Schurr, Olivier Celle
Přispěvatelé: Agence Française de Sécurité Sanitaire des Aliments (AFSSA)
Rok vydání: 2008
Předmět:
MESH: Sequence Analysis
DNA
Cancer Research
Chronic bee paralysis virus
Virus Replication
Polymerase Chain Reaction
Genome
MESH: Bees
law
MESH: Reverse Transcriptase Polymerase Chain Reaction
MESH: Animals
Mites
Reverse Transcriptase Polymerase Chain Reaction
Bees
Camponotus vagus
Infectious Diseases
MESH: RNA
Viral
Varroa destructor
behavior and behavior mechanisms
RNA
Viral
Apis mellifera
MESH: Genome
Viral
MESH: RNA Viruses
Chronic bee paralysis virus (CBPV)
Apiary
MESH: Paralysis
MESH: Ants
MESH: Mites
Insect Viruses
Genome
Viral
Biology
Quantitation
Species Specificity
Formica rufa
Virology
law.legal_case
MESH: Species Specificity
Animals
Paralysis
RNA Viruses
Ants
MESH: Chronic Disease
MESH: Virus Replication
fungi
RNA
MESH: Insect Viruses
MESH: Polymerase Chain Reaction
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Sequence Analysis
DNA
biology.organism_classification
Viral replication
Chronic Disease
Minus-strand-specific RT-PCR
Zdroj: Virus Research
Virus Research, Elsevier, 2008, 133 (2), pp.280-4. ⟨10.1016/j.virusres.2007.12.011⟩
ISSN: 0168-1702
DOI: 10.1016/j.virusres.2007.12.011
Popis: International audience; Detection of Chronic bee paralysis virus (CBPV) is reported for the first time in two species of ants (Camponotus vagus and Formica rufa) and in Varroa destructor. A quantitative real-time PCR (qPCR) method was used to detect and quantify CBPV in infected bees, ants and mites. A minus-strand-specific RT-PCR was used to assess viral replication. These results suggest a new way by which the infection may be spread and other sites of viral persistence in the close apiary environment.
Databáze: OpenAIRE
Externí odkaz: