Development of Chemical and Metabolite Sensors for Rhodococcus opacus PD630
| Autor: | Tae Seok Moon, Drew M. DeLorenzo, William R. Henson |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Bioconversion Nitrogen Metabolite Biomedical Engineering Biomass Promoter General Medicine Biosensing Techniques Biology biology.organism_classification Biochemistry Genetics and Molecular Biology (miscellaneous) Lignin 03 medical and health sciences chemistry.chemical_compound Rhodococcus opacus 030104 developmental biology chemistry Biochemistry Gene expression Rhodococcus Promoter Regions Genetic Bacteria |
| Zdroj: | ACS synthetic biology. 6(10) |
| ISSN: | 2161-5063 |
| Popis: | Rhodococcus opacus PD630 is a nonmodel, Gram-positive bacterium that possesses desirable traits for biomass conversion, including consumption capabilities for lignocellulose-based sugars and toxic lignin-derived aromatic compounds, significant triacylglycerol accumulation, relatively rapid growth rate, and genetic tractability. However, few genetic elements have been directly characterized in R. opacus, limiting its application for lignocellulose bioconversion. Here, we report the characterization and development of genetic tools for tunable gene expression in R. opacus, including: (1) six fluorescent reporters for quantifying promoter output, (2) three chemically inducible promoters for variable gene expression, and (3) two classes of metabolite sensors derived from native R. opacus promoters that detect nitrogen levels or aromatic compounds. Using these tools, we also provide insights into native aromatic consumption pathways in R. opacus. Overall, this work expands the ability to control and characterize gene expression in R. opacus for future lignocellulose-based fuel and chemical production. |
| Databáze: | OpenAIRE |
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