Physiological concentrations of β-hydroxybutyrate do not promote adipocyte browning
| Autor: | Gabriela Boltes Reis, Flaviane de Fatima Silva, Sandra Andreotti, Ayumi Cristina Medeiros Komino, Marcelo A. Christoffolete, Rogerio Sertie, Fábio Bessa Lima, Rennan de Oliveira Caminhotto |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Adipose tissue White adipose tissue 030226 pharmacology & pharmacy General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Adipose Tissue Brown 3T3-L1 Cells Internal medicine Adipocyte Brown adipose tissue medicine Browning Animals Citrate synthase Rats Wistar General Pharmacology Toxicology and Pharmaceutics Uncoupling Protein 1 3-Hydroxybutyric Acid biology Chemistry PERDA DE PESO General Medicine Metabolism Thermogenin Rats 030104 developmental biology Endocrinology medicine.anatomical_structure biology.protein |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0024-3205 |
| Popis: | Aims Previous work has demonstrated that ketogenic diets promote white fat browning; however, the exact mechanisms underlying this phenomenom have yet to be elucidated. Recently, an in vitro study showed that supraphysiological concentrations of β-hydroxybutyrate (βHB) had a strong influence on the induction of adipocyte browning. On the other hand, concentrations in the physiological range, achieved through ketogenic diets and prolonged fasting produce values of 1–3 mM and 4–7 mM, respectively. Herein, we investigated the impact of physiological concentrations of βHB on metabolism, and the expression of uncoupling protein 1 (UCP1) and other browning markers in adipose tissues. Main methods The effects of βHB on adipocyte browning were investigated in vitro, using primary cultures of isolated visceral and subcutaneous fat cells and cultured 3T3-L1 adipocytes, and in vivo. Key findings It was determined that βHB failed to induce changes in the oxidative capacity, citrate synthase activity or browning gene expression patterns in isolated adipocytes, and did not exert a permissive effect on β-adrenergic agonist-induced browning. In addition, 3T3-L1 adipocytes differentiated following βHB treatment exhibited downregulated Ucp1 expression levels, a result that was recapitulated in the subcutaneous adipose tissue of Wistar rats after βHB salt treatment. Rats administered βHB salts also presented reduced brown adipose tissue UCP1 protein expression. Significance The mechanisms underlying ketogenic diet-induced browning of adipocytes are not known. The results from the present study indicate that physiological concentrations of βHB are not responsible for this phenomenon, despite the observed βHB-mediated downregulation of UCP1 expression. |
| Databáze: | OpenAIRE |
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