Development of amoxicillin enzyme-linked immunosorbent assay and measurements of tissue amoxicillin concentrations in a pigeon microdialysis model
| Autor: | C.-H. Liu, J. P. Chan, Chi-Chung Chou, J.-P. Kao, B.-W. Koh, L.-C. Yeh, W.-M. Lee |
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| Rok vydání: | 2008 |
| Předmět: |
Microdialysis
Chromatography Dose-Response Relationship Drug Chemistry Cefazolin Amoxicillin Enzyme-Linked Immunosorbent Assay General Medicine Urine Cross Reactions Dicloxacillin Blood proteins Sensitivity and Specificity Drug Residues Cloxacillin Pharmacokinetics Area Under Curve Cefadroxil medicine Animals Animal Science and Zoology Columbidae Muscle Skeletal medicine.drug |
| Zdroj: | Poultry science. 87(3) |
| ISSN: | 0032-5791 |
| Popis: | A sensitive ELISA was developed for the detection of amoxicillin (AMX) in serum, urine, and milk. The ELISA used an indirect competitive method produced by coating the plate with ovalbumin conjugated with AMX hapten. Antibodies against AMX-BSA were detected by a goat-antirabbit antibody conjugated with peroxidase. Calibration standard curves ranged from 1.28 ng/mL to 20 microg/mL [IC(50) (inhibition concentration 50%) = 100 ng/mL], and the limits of detection were 1.3, 2.7, and 4.8 ng/mL for urine, milk, and serum, respectively. The intra- and interassay variations were less than 4 and 9.6%. The antibody produced against AMX cross-reacted highly with penicillin G (77%); cross-reacted moderately with ampicillin, oxacillin, and cloxacillin (56.9, 51.4, and 48.8%, respectively); but was considered non-cross-reactive with dicloxacillin (7.4%), cefadroxil ( |
| Databáze: | OpenAIRE |
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