Therapeutic effects of vaccine derived from amastigote surface protein-2 (ASP-2) against Chagas disease in mouse liver
| Autor: | Daniel Araki Ribeiro, Mariana Cruz Lazzarin, Flavia de Oliveira, José Fontes dos Santos, José Ronnie Vasconcelos, Oscar-Bruna Romero, Luciana Pellegrini Pisani, Flávia Andressa Pidone Ribeiro, Ricardo T. Gazzinelli, Camila Pontes |
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| Rok vydání: | 2019 |
| Předmět: |
Chagas Cardiomyopathy
Liver Cirrhosis Protozoan Vaccines 0301 basic medicine Chagas disease Trypanosoma cruzi Immunology Neuraminidase Nitric Oxide Synthase Type II Biology Biochemistry Adenoviridae Mice 03 medical and health sciences 0302 clinical medicine Immune system Gene expression medicine Animals Immunology and Allergy Amastigote Molecular Biology Caspase 3 Cytochromes c Hematology medicine.disease biology.organism_classification Toll-Like Receptor 4 Vaccination Interleukin 10 030104 developmental biology Liver Cyclooxygenase 2 030220 oncology & carcinogenesis TLR4 Cytokines Female |
| Zdroj: | Cytokine. 113:285-290 |
| ISSN: | 1043-4666 |
| DOI: | 10.1016/j.cyto.2018.07.017 |
| Popis: | This study investigated the efficacy of the vaccine in liver of mice infected with the Trypanosoma cruzi ( T. cruzi ) and immunized with AdASP-2. For this purpose, histopathological analysis and gene expression of COX-2, TNF-alpha, TNFR, iNOS, cytochrome C , caspase-3, TLR4, IL-6 and IL10 were evaluated. The following groups were used in this study: Group 1 - Control Group (CTRL) animals received AdβGal vehicle; Group 2 - Infected Group (TC) animals were infected with T. cruzi ; Group 3 - Immunized Group (AdASP-2): animals were immunized by AdASP-2 vaccine; Group 4 - Immunized and Infected Group (AdASP-2+TC) animals were infected with T. cruzi and immunized by AdSP-2 vaccine. A significant decrease of amastigote nests was noticed in the group of animals that were immunized with AdASP-2 and infected on the same day. COX-2 and TNF-alpha gene expressions increased in TC group, whereas TNF-alpha decreased in the TC+AdASP-2 group. TNFR expression was high in AdASP-2+TC group. iNOS expression was high for all experimental groups whereas cytochrome C decreased for all experimental groups. Caspase 3 increased in TC and TC+AdASP-2 groups. The gene expression of TLR4 and IL-10 showed an increase in AdASP-2+TC group. Finally, hepatic fibrosis was noticed to TC and AdASP-2 + TC groups. Taken together, our results demonstrated that vaccination with AdASP-2 was effective against the acute phase of experimental Chagas disease as a result of a more powerful and rapid immune response closely related to expression of some inflammatory genes, such as iNOS, TNF-alpha, TLR 4, and IL-10. |
| Databáze: | OpenAIRE |
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