A MAP kinase is activated late in plant mitosis and becomes localized to the plane of cell division

Autor: Neville S. Huskisson, Markus Mattauch, Ornella Calderini, László Bögre, Erwin Heberle-Bors, Pavla Binarová, Heribert Hirt, Christina Pollaschek, Stefan Kiegerl, Sandra Till, Patrick J. Barker, Claudia Jonak
Rok vydání: 1999
Předmět:
Cell division
Molecular Sequence Data
Fluorescent Antibody Technique
Mitosis
Cell Cycle Proteins
Plant Science
Post-Translational/physiology Sequence Homology
Biology
Phragmoplast
Microtubules
Amino Acid Sequence
Telophase
Cloning
Molecular
Anaphase
Plant Proteins
Sequence Homology
Amino Acid
Molecular Fluorescent Antibody Technique Medicago sativa/*genetics/metabolism Microtubules/metabolism Mitogen-Activated Protein Kinases/*genetics/metabolism Mitosis/*genetics/physiology Molecular Sequence Data *Plant Proteins Protein Processing
Amino Acid Sequence Cell Cycle Proteins/genetics/metabolism Cell Division/genetics/physiology Cloning
Cell Biology
Cell plate
Faculty of Science\Biological Science
Cell cycle
Cell biology
Amino Acid
Mitogen-Activated Protein Kinases
Protein Processing
Post-Translational
Cytokinesis
Cell Division
Medicago sativa
Research Article
Zdroj: Plant Cell, 11 (1)
ISSN: 1040-4651
Popis: In eukaryotes, mitogen-activated protein kinases (MAPKs) are part of signaling modules that transmit diverse stimuli, such as mitogens, developmental cues, or various stresses. Here, we report a novel alfalfa MAPK, Medicago MAP kinase 3 (MMK3). Using an MMK3-specific antibody, we detected the MMK3 protein and its associated activity only in dividing cells. The MMK3 protein could be found during all stages of the cell cycle, but its protein kinase activity was transient in mitosis and correlated with the timing of phragmoplast formation. Depolymerization of microtubules by short treatments with the drug amiprophosmethyl during anaphase and telophase abolished MMK3 activity, indicating that intact microtubules are required for MMK3 activation. During anaphase, MMK3 was found to be concentrated in between the segregating chromosomes; later, it localized at the midplane of cell division in the phragmoplast. As the phragmoplast microtubules were redistributed from the center to the periphery during telophase, MMK3 still localized to the whole plane of division; thus, phragmoplast microtubules are not required to keep MMK3 at this location. Together, these data strongly support a role for MMK3 in the regulation of plant cytokinesis.
Databáze: OpenAIRE
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