T lymphocytes in patients with primary vasculitis: expansion of CD8+ T cells with the propensity to activate polymorphonuclear neutrophils
| Autor: | Ellen Bleck, Matthias Schneider, C Iking-Konert, Benedikt Ostendorf, T. Vogl, K. Andrassy, Oliver Sander, C. Wagner, G. M. Hänsch, B. Prior |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Vasculitis
Neutrophils Myeloblastin Receptor expression Population CD8-Positive T-Lymphocytes Lymphocyte Activation Neutrophil Activation Antibodies Antineutrophil Cytoplasmic Immunophenotyping Interferon-gamma CD28 Antigens Rheumatology Humans Medicine Cytotoxic T cell Pharmacology (medical) Lymphocyte Count education Cells Cultured Cell Proliferation Anti-neutrophil cytoplasmic antibody MHC class II education.field_of_study CD11b Antigen biology business.industry Granulomatosis with Polyangiitis CD28 T lymphocyte Case-Control Studies Immunology biology.protein business Biomarkers CD8 |
| Zdroj: | Rheumatology. 47:609-616 |
| ISSN: | 1462-0332 1462-0324 |
| DOI: | 10.1093/rheumatology/ken028 |
| Popis: | Objectives. To gain insight into the immune pathogenesis of primary ANCA-associated vasculitides, the prevalence of circulating T lymphocytes expressing CD11b as a marker for activation was analysed in patients with WG or microscopic polyangiitis. Methods. Receptor expression and IFNsynthesis were measured in T cells of patients with active disease by cytofluorometry and compared with expression in patients in remission and in healthy donors. Results. During active disease, a small but conspicuous population of CD8þCD28þCD11bþ was found which produced IFN� . In healthy donors and in patients in remission or undergoing immunosuppressive therapy, CD11b was exclusively associated with CD8þCD28� cells, the latter being more frequent in patients with long-lasting or severe disease. In vitro experiments confirmed that CD11b is up-regulated when T cells are activated. After multiple rounds of restimulation, the CD11b expression persists whereas CD28 expression is lost, compatible with the notion that CD8þCD28þCD11bþ represents a transient phenotype in the course of T-cell activation. The IFN� -producing T cells activated polymorphonuclear neutrophils (PMN) to express MHC class II, thus generating the same PMN phenotype as in patients with active ANCA-associated vasculitis. A similar PMN phenotype could be generated by cultivation with supernatants of activated T cells or by IFN� alone, but not by antibodies to proteinase 3. Conclusions. In active primary vasculitis, a small population of CD8þ T cells, identified by the expression of CD11b, expands, producing IFN� . These T cells could activate PMN, thus generating a long-living and potentially destructive PMN phenotype. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|