A method for the selection of production media for actinomycete strains based on their metabolite HPLC profiles
| Autor: | Juan B. García, Julia Feliz, M. Teresa Díez, Aurora Mira, Pilar Hernández, José R. Tormo, Mar DeAntonio, Fernando Pelaez |
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| Rok vydání: | 2002 |
| Předmět: |
Chromatography
biology Drug Industry Metabolite Microorganism Bioengineering Secondary metabolite biology.organism_classification Classification Applied Microbiology and Biotechnology High-performance liquid chromatography Culture Media Actinobacteria chemistry.chemical_compound Industrial Microbiology chemistry Chemical diversity Fermentation medicine Bacteria Selection (genetic algorithm) Chromatography High Pressure Liquid Biotechnology medicine.drug |
| Zdroj: | Journal of industrial microbiologybiotechnology. 30(10) |
| ISSN: | 1367-5435 |
| Popis: | The manipulation of growth conditions of microorganisms is a common strategy used by pharmaceutical companies to improve the quantities and spectra of secondary metabolites with potential therapeutic interest. In this work, the effects of fermentation media on secondary metabolite production from a set of Actinomycetes was statistically compared. For this purpose, we created an automated method for comparing the ability of microorganisms to produce different secondary metabolites. HPLC analyses guided the selection of those media in which a wider chemical diversity was obtained from microorganisms inoculated in a wide spectrum of production media. Fermented media yielding a better secondary metabolite profile were included in subsequent drug discovery screening. |
| Databáze: | OpenAIRE |
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