St. John's Wort versus Counterfeit St. John's Wort: An HPTLC Study
| Autor: | E Reich, Ben Lucas, Anton Bzhelyansky, D Frommenwiler, Sidney Sudberg, Maged H. M. Sharaf |
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| Rok vydání: | 2016 |
| Předmět: |
Pharmacology
Chromatography biology Plant Extracts Chemistry Hypericum perforatum biology.organism_classification Analytical Chemistry Counterfeit 03 medical and health sciences 0302 clinical medicine Counterfeit Drugs 030220 oncology & carcinogenesis White light Environmental Chemistry Scanning densitometry Hypericum Agronomy and Crop Science Chromatography High Pressure Liquid 030217 neurology & neurosurgery Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 99:1204-1212 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.5740/jaoacint.16-0170 |
| Popis: | Hypericum perforatum L. is the most commonly used herb for treating depression. Due to the popularity of this botanical, there is a potential for economically driven adulteration of St. John's wort (SJW) products. The goal of this study was to investigate SJW ingredients suspected to be adulterated based on simple preliminary HPTLC tests. Commercial samples were analyzed by HPTLC following the United States Pharmacopeia (USP) monograph methodology, with additional visualization under white light. A number of these samples presented odd methanolic solution colors and unconventional HPTLC fingerprints, suggesting the presence of other species and/or extraneous polar additives. To achieve identification and separation of the polar additives, a new reversed-phase HPTLC method was developed. The adulterants were identified as synthetic dyes in the amounts of 0.51 to 1.36% by weight. Identities of the dyes were confirmed by scanning densitometry and HPTLC-MS. A modified USP method with additional detection mode permitted the identification of eight SJW samples adulterated with dyes and six others with flavonoid fingerprints different from those specified by USP from a total of 37 samples of dry extracts, finished products, and bulk raw herb. A decision flowchart is proposed to guide the detection of adulteration of SJW in a systematic fashion. |
| Databáze: | OpenAIRE |
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