Chemokine secretion of human cells in response to Toxoplasma gondii infection
| Autor: | Carolyn F. Denney, Sharon L. Reed, Lars Eckmann |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Chemokine
medicine.medical_treatment Immunology Inflammation Biology Microbiology Proinflammatory cytokine medicine Animals Humans Secretion Interleukin 8 RNA Messenger Cells Cultured Interleukin-8 Toxoplasma gondii Fibroblasts biology.organism_classification Infectious Diseases Cytokine Chemokine secretion biology.protein Parasitology medicine.symptom Chemokines Fungal and Parasitic Infections HT29 Cells Toxoplasma HeLa Cells Interleukin-1 |
| Zdroj: | Infection and immunity. 67(4) |
| ISSN: | 0019-9567 |
| Popis: | The ubiquitous protozoan parasite Toxoplasma gondii is a major cause of morbidity and mortality in neonates and immunocompromised hosts. Both acute invasion and reactivation of latent infection result in an inflammatory reaction with lymphocytes, macrophages, and neutrophils. The mechanisms responsible for triggering the local host response to toxoplasmosis are not fully understood. Infection of monolayers of human HeLa epithelial cells and fibroblasts with T. gondii resulted in a marked increase in the expression of interleukin-8 (IL-8)-specific mRNA and secretion of the proinflammatory and chemoattractant cytokines interleukin-8 (IL-8), GROα, and MCP-1. Host cell invasion and lysis were required for this response, as tachyzoite lysates alone had no effect on IL-8 secretion. IL-8 release was dependent on the release of soluble host cell factors: IL-1α in HeLa cells and an additional mediator in fibroblasts. HT-29 epithelial cells, which lack IL-1α or another IL-8-inducing activity, did not release IL-8 after infection, although they were efficiently infected with T. gondii and increased IL-8 secretion in response to added IL-1α. These data suggest that proinflammatory chemokine secretion is an important host cell response to toxoplasmosis and that the release of IL-1α and other mediators from lysed host cells is critical for this chemokine response. |
| Databáze: | OpenAIRE |
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