Epidermal growth factor receptor-targeted immune magnetic liposomes capture circulating colorectal tumor cells efficiently
| Autor: | Jing-Yu Zhang, Xiao-Zhen Hu, Ai-Jun Zhang, Kang-Kang Wu, Zheng-Feng Chen, Qing Wang, Jing-Hua Kuai |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Colorectal cancer Sensitivity and Specificity Antibodies 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Circulating tumor cell Immune system Epidermal growth factor Antigens Neoplasm Cell Line Tumor medicine Humans Immune magnetic liposomes Epidermal growth factor receptor Colorectal tumor Liposome integumentary system biology Immunomagnetic Separation Gastroenterology Circulating tumor cells Epithelial cell adhesion molecule General Medicine biochemical phenomena metabolism and nutrition Basic Study equipment and supplies medicine.disease Epithelial Cell Adhesion Molecule Neoplastic Cells Circulating Microspheres ErbB Receptors 030104 developmental biology Treatment Outcome chemistry 030220 oncology & carcinogenesis Liposomes Cancer research biology.protein Nanoparticles Colorectal Neoplasms human activities HT29 Cells |
| Zdroj: | World Journal of Gastroenterology |
| ISSN: | 2219-2840 |
| Popis: | AIM To compare the capacity of newly developed epidermal growth factor receptor (EGFR)-targeted immune magnetic liposomes (EILs) vs epithelial cell adhesion molecule (EpCAM) immunomagnetic beads to capture colorectal circulating tumor cells (CTCs). METHODS EILs were prepared using a two-step method, and the magnetic and surface characteristics were confirmed. The efficiency of capturing colorectal CTCs as well as the specificity were compared between EILs and EpCAM magnetic beads. RESULTS The obtained EILs had a lipid nanoparticle structure similar to cell membrane. Improved binding with cancer cells was seen in EILs compared with the method of coupling nano/microspheres with antibody. The binding increased as the contact time extended. Compared with EpCAM immunomagnetic beads, EILs captured more CTCs in peripheral blood from colorectal cancer patients. The captured cells showed consistency with clinical diagnosis and pathology. Mutation analysis showed same results between captured CTCs and cancer tissues. CONCLUSION EGFR antibody-coated magnetic liposomes show high efficiency and specificity in capturing colorectal CTCs. |
| Databáze: | OpenAIRE |
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