Measurement of cholesterol 7 alpha-hydroxylase activity with selected ion monitoring
| Autor: | Ajit Sanghvi, E Grassi, M. Galli Kienle, R Lester, G. Galli, C Bartman |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
chemistry.chemical_classification
Chromatography Cholesterol Extraction (chemistry) Analytical chemistry Substrate (chemistry) QD415-436 Cell Biology Cholesterol 7 alpha-hydroxylase Biochemistry chemistry.chemical_compound Endocrinology Enzyme chemistry Yield (chemistry) Microsome lipids (amino acids peptides and proteins) Selected ion monitoring |
| Zdroj: | Journal of Lipid Research, Vol 22, Iss 4, Pp 720-724 (1981) |
| ISSN: | 0022-2275 |
| Popis: | Formation of 7 alpha-hydroxycholesterol by rat liver microsomes was quantitated using a gas chromatograph-mass spectrometer operated in selected ion monitoring mode. This procedure measures the mass of 7 alpha-hydroxycholesterol formed and does not require addition of labeled cholesterol to monitor the reaction, since endogenous microsomal cholesterol serves as substrate for the enzyme cholesterol 7 alpha-hydroxylase. The method requires only the extraction of lipids from microsomal incubations and injection of a small fraction of this extract into the apparatus. Microsomes from normal rat livers incubated for different periods were found to yield increased 7 alpha-hydroxycholesterol with time. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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