Prevalence of hepatitis delta virus among chronic hepatitis B carriers in a large tertiary center in the Netherlands
| Autor: | Boris J. B. Beudeker, Corine H. GeurtsvanKessel, Annemiek A. van der Eijk, Jolanda J.C. Voermans, Tamara Kuhlemann, Andre Boonstra, Robert J. de Knegt |
|---|---|
| Přispěvatelé: | Gastroenterology & Hepatology, Virology |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Hepatitis B virus Genotype viruses 030106 microbiology Viremia Iran Serology 03 medical and health sciences 0302 clinical medicine Hepatitis B Chronic SDG 3 - Good Health and Well-being Virology Epidemiology Prevalence Medicine Outpatient clinic Humans 030212 general & internal medicine Genotyping Phylogeny Netherlands Hepatitis B Surface Antigens business.industry virus diseases Reproducibility of Results Hepatitis B biochemical phenomena metabolism and nutrition medicine.disease Hepatitis D Infectious Diseases Hepatitis Delta Virus business Viral hepatitis |
| Zdroj: | Journal of Clinical Virology Journal of Clinical Virology, 141:104870. Elsevier |
| ISSN: | 1386-6532 |
| DOI: | 10.1016/j.jcv.2021.104870 |
| Popis: | Background & aims Hepatitis D virus infection (HDV) is considered the most severe form of viral hepatitis. In this study, we aimed to evaluate the prevalence of HDV infection in a tertiary center of a large, multi-ethnic city in the Netherlands. Moreover, we validate the reliability of a novel anti-HDV CLIA assay. Methods All HBsAg-positive patients visiting the outpatient clinic between 2017 and 2019 were tested for HDV serology. Seropositive serum samples were further assessed by HDV RNA PCR and Sanger sequencing to identify the HDV genotype. Results The CLIA assay was 100% sensitive and 98% specific. Out of 925 patients 3.7% tested seropositive for HDV, and HDV viremia was confirmed in 2.0%. The majority of patients had a non-Dutch background and did not speak English or Dutch. We detected HDV genotype 5 (N = 3), and genotype 1 (N = 15). Phylogenetic analysis demonstrated HDV1 clusters composed of sub-Saharan Africa isolates, central Asian, Turkish, Iranian and European isolates. Conclusions The prevalence of HDV infection in a tertiary center in the Netherlands was 2.0% among HBsAg-positive individuals, and mainly in non-Dutch individuals. Only HDV genotype 1 and 5 isolates were detected, which was found to match with the patient's country of origin. |
| Databáze: | OpenAIRE |
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